Laboratory of Analytical Toxicology, Feevale University, Novo Hamburgo, Brazil.
National Institute of Forensic Science and Technology, Brazil.
Biomed Chromatogr. 2020 Dec;34(12):e4952. doi: 10.1002/bmc.4952. Epub 2020 Aug 6.
The detection of the markers of Cannabis consumption in biological specimens is an important task for drug testing laboratories in varous contexts. A simple assay combining salting-out assisted liquid-liquid extraction sample preparation and LC-MS/MS analysis was applied to the measurement of Δ -tetrahydrocannabinol, 11-nor-9-carboxy-Δ -tetrahydrocannabinol (THC-COOH), 11-hydroxy-Δ -tetrahydrocannabinol, cannabinol and cannabidiol concentrations in 100 μl plasma specimens. The assay had linearity of 1-100 ng ml for THC-COOH and 0.5-50 ng ml for the other tested cannabinoids. Assay validation criteria were fulfilled. Extraction yields (88.7-97.3%) and internal-standard correct matrix effects (-9.6 to +5.4%) were acceptable. The assay was applied to 238 clinical specimens from trauma patients, with 19 samples presenting quantifiable concentrations of at least one of the target compounds. The developed assay is a simple and efficient strategy for simultaneous measurement of Δ -tetrahydrocannabinol, THC-COOH, 11-hydroxy-Δ -tetrahydrocannabinol, cannabinol and cannabidiol concentrations in plasma specimens.
检测生物样本中大麻使用的标志物是各种情况下药物检测实验室的一项重要任务。本研究应用了一种简单的分析方法，结合了盐析辅助液液萃取样品制备和 LC-MS/MS 分析，用于测量 100μl 血浆样本中 Δ -四氢大麻酚、11-去甲-9-羧基-Δ -四氢大麻酚（THC-COOH）、11-羟基-Δ -四氢大麻酚、大麻酚和大麻二酚的浓度。该方法对 THC-COOH 的线性范围为 1-100ng/ml，对其他测试大麻素的线性范围为 0.5-50ng/ml。符合分析方法验证标准。提取回收率（88.7-97.3%）和内标正确的基质效应（-9.6 至+5.4%）是可接受的。该方法应用于 238 例创伤患者的临床样本，其中 19 个样本中至少有一种目标化合物的浓度可定量检测。所开发的方法是一种简单有效的策略，用于同时测量血浆样本中 Δ -四氢大麻酚、THC-COOH、11-羟基-Δ -四氢大麻酚、大麻酚和大麻二酚的浓度。
