一种用于分析小剂量大麻提取物后人体乙二胺四乙酸血浆和尿液中大麻素的液相色谱-串联质谱法的开发。
Development of a LC/MS/MS method for the analysis of cannabinoids in human EDTA-plasma and urine after small doses of Cannabis sativa extracts.
作者信息
Grauwiler Sandra B, Scholer André, Drewe Jürgen
机构信息
Clinical Chemical Laboratory, Toxicology Section, University Hospital, Petersgraben 4, 4031 Basel, Switzerland.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 1;850(1-2):515-22. doi: 10.1016/j.jchromb.2006.12.045. Epub 2007 Jan 11.
A novel high-performance liquid chromatographic separation method with tandem-mass spectrometry detection was developed for the simultaneous determination of Delta(9)-tetrahydrocannabinol (THC) and its major metabolites 11-hydroxy-Delta(9)-tetrahydrocannabinol (11-OH-THC) and 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) as well as the components cannabidiol (CBD) and cannabinol (CBN) in human EDTA-plasma and urine. Run time was 25 min. Lower limit of quantification was 0.2 ng/ml. The coefficients of variation of all inter- and intra-assay determinations were between 1.3 and 15.5%. The method was successfully applied to the determination of cannabinoids in human plasma and human urine after administration of Delta(9)-tetrahydrocannabinol or Cannabis sativa extracts.
开发了一种采用串联质谱检测的新型高效液相色谱分离方法，用于同时测定人乙二胺四乙酸（EDTA）血浆和尿液中的Δ⁹-四氢大麻酚（THC）及其主要代谢物11-羟基-Δ⁹-四氢大麻酚（11-OH-THC）和11-去甲-Δ⁹-四氢大麻酚-9-羧酸（THC-COOH），以及大麻二酚（CBD）和大麻酚（CBN）成分。运行时间为25分钟。定量下限为0.2纳克/毫升。所有批间和批内测定的变异系数在1.3%至15.5%之间。该方法成功应用于给予Δ⁹-四氢大麻酚或大麻提取物后人体血浆和尿液中大麻素的测定。
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