Department of Medical, Surgical and Neurological Science, Dermatology Section, University of Siena, S. Maria alle Scotte Hospital, Siena, Italy.
Department of Surgical Sciences and Integrated Diagnostics, San Martino University General Hospital, Genoa, Italy.
J Biophotonics. 2020 Nov;13(11):e202000179. doi: 10.1002/jbio.202000179. Epub 2020 Aug 13.
We report the first series of melanomas (MMs) where the surgical margins were evaluated both by ex vivo confocal microscopy (EVCM) and in vivo reflectance confocal microscopy (RCM). We evaluated the surgical margins of 42 cutaneous MMs of lentigo maligna/lentigo maligna melanoma type and 2 mucosal MMs with EVCM. Cutaneous MMs also underwent RCM mapping. Imaging results were compared with histopathology. The rate of correct identification of the tumor margins (invaded or not invaded) was 97.6% for RCM (evaluations of cutaneous MMs) and 95.5% for EVCM (evaluations of both cutaneous and mucosal MMs). Our study showed that the MM extension is visible under EVCM and that the combination of in vivo RCM and EVCM can be a new strategy for the evaluation of surgical margins of MMs.
我们报告了第一批通过离体共聚焦显微镜 (EVCM) 和体内反射共聚焦显微镜 (RCM) 评估手术切缘的黑色素瘤 (MM)。我们使用 EVCM 评估了 42 例交界痣/恶性交界痣型和 2 例黏膜 MM 的皮肤 MM 的手术切缘。皮肤 MM 还进行了 RCM 定位。将影像学结果与组织病理学进行比较。对于 RCM（评估皮肤 MM），正确识别肿瘤边界（侵犯或未侵犯）的比率为 97.6%，对于 EVCM（评估皮肤和黏膜 MM），正确识别肿瘤边界的比率为 95.5%。我们的研究表明，EVCM 下可见 MM 延伸，体内 RCM 和 EVCM 的联合应用可以成为评估 MM 手术切缘的新策略。
