Wang Yuanyuan, Maruthamuthu Murali Kannan, Jeong Jaehoon, Yoo Ik-Keun, Kim Tae Wan, Hong Soon Ho
Department of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan 44610, Republic of Korea.
Department of Biotechnology & Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea.
J Biotechnol. 2020 Oct 10;322:90-95. doi: 10.1016/j.jbiotec.2020.07.014. Epub 2020 Jul 22.
In this study, constructed Escherichia coli could efficiently adsorb fenitrothion by displaying a pesticide-binding peptide on it using the anchoring motif OmpC. A codon-optimized, pesticide-binding peptide was attached to the C-terminus of OmpC at loop 7 (993 bp). The efficiency of fenitrothion binding by the monomer peptide was evaluated under different temperatures, pH levels, and fenitrothion concentrations. To enhance fenitrothion adsorption, a dimer of pesticide-binding peptide was also constructed and displayed. Compared with the peptide monomer, the dimer-displaying strain showed superior fenitrothion-binding ability. The performance of the strains was evaluated in artificial polluted soil, and their morphology was analyzed by FE-SEM. The results showed that these two kinds of constructed strains can adsorb fenitrothion in contaminated environments with no cellular activity reduction. ARTICLE INFO.
在本研究中，构建的大肠杆菌通过使用锚定基序OmpC在其上展示一种农药结合肽，能够高效吸附杀螟硫磷。一个经过密码子优化的农药结合肽在第7环（993 bp）处连接到OmpC的C末端。在不同温度、pH值和杀螟硫磷浓度下评估了单体肽对杀螟硫磷的结合效率。为了增强杀螟硫磷的吸附，还构建并展示了农药结合肽的二聚体。与肽单体相比，展示二聚体的菌株表现出更强的杀螟硫磷结合能力。在人工污染土壤中评估了这些菌株的性能，并通过场发射扫描电子显微镜（FE-SEM）分析了它们的形态。结果表明，这两种构建的菌株能够在污染环境中吸附杀螟硫磷，且细胞活性没有降低。文章信息。
