Ao Fen, Shen Wen, Ge Xuemei, Wang Lan, Ning Yuanlan, Ren Huijun, Fan Guodong, Huang Menghui
School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, PR China.
School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, PR China.
Colloids Surf B Biointerfaces. 2020 Nov;195:111264. doi: 10.1016/j.colsurfb.2020.111264. Epub 2020 Jul 17.
The quercetin loaded Eudragit L-100 nanofiber membrane with high ductility and a desired drug release rate was prepared in this work. The morphological characteristics of the Eudragit L-100 nanofibers with different drug loadings amount were observed by scanning electron microscope (SEM). After adding Polyethylene glycol-4000 (PEG-4000), the degree of the fiber breakage decreased and the fiber length increased. Fiber diameter analysis, X-ray diffraction (XRD), thermal analysis (TA) and differential scanning calorimetry (DSC) have demonstrated that the crystallinity of the fiber membrane was significantly reduced after adding PEG-4000. The mechanical property test also showed that the fiber membrane with PEG-4000 had a greater elongation at break. The in vitro release experiments showed that after adding PEG-4000, the drug-loaded fibers showed rapid release at a pH of 7.4. After adopting the strategy of reducing the crystallinity, the ductility of the fiber was enhanced, which could provide a fesibility to enable this nanofiber membrane to be used in sports wound healing treatment. The electrospun Eudragit L-100 nanofiber membranes loaded with quercetin have the potential to be applied in sport wound healing of skin, tissue and joints.
本研究制备了具有高延展性和理想药物释放速率的载槲皮素Eudragit L-100纳米纤维膜。通过扫描电子显微镜（SEM）观察了不同载药量的Eudragit L-100纳米纤维的形态特征。添加聚乙二醇-4000（PEG-4000）后，纤维断裂程度降低，纤维长度增加。纤维直径分析、X射线衍射（XRD）、热分析（TA）和差示扫描量热法（DSC）表明，添加PEG-4000后纤维膜的结晶度显著降低。力学性能测试还表明，含有PEG-4000的纤维膜具有更大的断裂伸长率。体外释放实验表明，添加PEG-4000后，载药纤维在pH值为7.4时表现出快速释放。采用降低结晶度的策略后，纤维的延展性增强，这为使这种纳米纤维膜用于运动伤口愈合治疗提供了可行性。电纺载槲皮素Eudragit L-100纳米纤维膜具有应用于皮肤、组织和关节运动伤口愈合的潜力。
