Özer Sinan, Akyıl Evrim, Arslan Rana, Arı Neziha Senem
Faculty of Pharmacy, Department of Pharmaceutical Technology, Anadolu University, Eskisehir 26470, Turkey.
Faculty of Pharmacy, Department of Pharmacology, Anadolu University, Eskisehir 26470, Turkey.
ACS Omega. 2025 Jun 30;10(27):29037-29058. doi: 10.1021/acsomega.5c01101. eCollection 2025 Jul 15.
Wound healing is a dynamic and multifactorial process that can be significantly impaired by oxidative stress, microbial infection, and chronic systemic conditions, often resulting in delayed recovery and poor tissue regeneration. This study investigates the use of rutin hydrate, a bioactive flavonoid with antioxidant and collagen-promoting effects, in electrospun nanofiber dressings to enhance wound repair outcomes. Electrospinning technology was used to fabricate nanofibers from poly-(vinyl alcohol) (PVA) and Eudragit L100, with optimized parameters determined via the Taguchi method. The nanofibers had average diameters of 258.371 nm (PVA) and 125.115 nm (Eudragit), with drug loading capacities of 78.735 ± 2.307 μg/mg per mass and 87.983 ± 2.055 μg/cm per area (PVA); 76.833 ± 2.238 μg/mg per mass and 85.807 ± 1.502 μg/cm per area (Eudragit). Characterization via SEM, FTIR, DSC, and NMR confirmed uniform, bead-free nanofibers with enhanced stability and controlled drug release. In vitro studies showed first-order drug release kinetics (Hixson-Crowell model), balancing burst release with sustained delivery. In vivo wound healing in rats demonstrated significantly faster recovery with rutin-loaded nanofibers ( < 0.0001 for F-PVA-Rutin and F-EUD-Rutin on day 3 and day 7). Histological analysis revealed reduced neutrophil infiltration, enhanced granulation tissue, and improved angiogenesis, confirming the therapeutic efficacy of rutin. These findings support the potential of rutin-loaded electrospun nanofiber dressings as an effective and scalable approach for promoting wound healing through localized, sustained drug delivery.
伤口愈合是一个动态的多因素过程，氧化应激、微生物感染和慢性全身性疾病会严重损害这一过程，常常导致恢复延迟和组织再生不良。本研究调查了水合芦丁（一种具有抗氧化和促进胶原蛋白生成作用的生物活性类黄酮）在电纺纳米纤维敷料中的应用，以提高伤口修复效果。采用静电纺丝技术由聚（乙烯醇）（PVA）和聚丙烯酸树脂L100制备纳米纤维，并通过田口方法确定优化参数。纳米纤维的平均直径分别为258.371 nm（PVA）和125.115 nm（聚丙烯酸树脂），每质量的载药量为78.735±2.307μg/mg，每面积的载药量为87.983±2.055μg/cm²（PVA）；每质量的载药量为76.833±2.238μg/mg，每面积的载药量为85.807±1.502μg/cm²（聚丙烯酸树脂）。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、差示扫描量热法（DSC）和核磁共振（NMR）进行表征，证实纳米纤维均匀无珠，稳定性增强且药物释放可控。体外研究显示为一级药物释放动力学（希克森-克劳威尔模型），平衡了突释和持续释放。大鼠体内伤口愈合实验表明，载芦丁纳米纤维能显著加快恢复速度（第3天和第7天，F-PVA-芦丁和F-EUD-芦丁的P值均<0.0001）。组织学分析显示中性粒细胞浸润减少，并增强了肉芽组织和改善了血管生成，证实了芦丁的治疗效果。这些发现支持了载芦丁电纺纳米纤维敷料作为一种通过局部、持续药物递送促进伤口愈合的有效且可扩展方法的潜力。
