The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, China.
National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Key Laboratory of Tea Science of Ministry of Education, College of Horticulture, Hunan Agricultural University, Changsha, 410128, China; Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop., Hunan Agricultural University, Changsha, 410128, China.
Biomed Pharmacother. 2020 Oct;130:110514. doi: 10.1016/j.biopha.2020.110514. Epub 2020 Jul 21.
Processing of dark tea varieties, such as Fu brick tea, Liupao tea, Qianliang tea, and Qing brick tea, includes solid-state fermentation involving microorganisms. In this study, we analyzed the major chemical constituents of dark tea extracts and evaluated their modulatory effect on the gastrointestinal function in normal mice, including the improvement of gastrointestinal transit and intestinal microbial, as well as the attenuation of intestinal microbial dysbiosis and intestinal pathological damage, and the adjustment of immune function in antibiotic-treated mice. Substantial differences in major chemical constituents, including total polyphenols, total organic acids, water extract content, 18 free amino acids, gallic acid, and six tea catechins, were observed among Fu brick tea, Qianliang tea, Qing brick tea, and Liupao tea extracts. Extracts from the four dark tea varieties significantly promoted gastrointestinal transit and colonization of beneficial Bifidobacterium and Lactobacillus, and inhibited the growth of harmful Escherichia coli and Enterococcus in normal mice. In addition, Qianliang tea, Qing brick tea, and Liupao tea extracts significantly accelerated the reversal of the ampicillin sodium-induced pathological damage in the ileum, intestinal bacterial dysbiosis (Bifidobacterium, Lactobacillus, E. coli, and Enterococcus), and low immunity.
黑茶品种（如茯砖茶、六堡茶、千两茶和青砖茶）的加工包括涉及微生物的固态发酵。在本研究中，我们分析了黑茶提取物的主要化学成分，并评估了它们对正常小鼠胃肠道功能的调节作用，包括改善胃肠道转运和肠道微生物，减轻肠道微生物失调和肠道病理损伤，以及调节抗生素处理小鼠的免疫功能。茯砖茶、千两茶、青砖茶和六堡茶提取物的主要化学成分（包括总多酚、总有机酸、水提物含量、18 种游离氨基酸、没食子酸和六种茶儿茶素）存在显著差异。四种黑茶提取物均能显著促进胃肠道转运和有益双歧杆菌和乳酸杆菌的定植，并抑制正常小鼠有害大肠杆菌和肠球菌的生长。此外，千两茶、青砖茶和六堡茶提取物还能显著加速氨苄西林钠诱导的回肠病理损伤、肠道细菌失调（双歧杆菌、乳酸杆菌、大肠杆菌和肠球菌）和低免疫力的逆转。
