Ming Shengjin, Ou Jinxi, Huang Ying, Tang Zhongqing, Qin Yuechao, Ma Hongxi, Gan Siling, Li Zhongxia
Department of Clinical Laboratory, Wuzhou Gongren Hospital (The Seventh Affiliated Hospital of Guangxi Medical University), 1 Gaodi Road, Wuzhou, 543001, China.
Department of Gastroenterology, Wuzhou Gongren Hospital (The Seventh Affiliated Hospital of Guangxi Medical University), 1 Gaodi Road, Wuzhou, 543001, China.
Open Life Sci. 2025 May 20;20(1):20251106. doi: 10.1515/biol-2025-1106. eCollection 2025.
Liupao tea is known for its anti-inflammatory antioxidant and regulation of gut microecological balance properties. This study aims to investigate the therapeutic effects of Liupao tea aqueous extract (LPTAE) on ulcerative colitis (UC) induced by dextran sulfate sodium (DSS) in rats. The rats were randomly divided into five groups: the Normal group, the DSS group, the LPTL group, the LPTM group, and the LPTH group. Throughout the experiment, the rats' activity levels, stool consistency, and body weights were observed and recorded daily. After the experiment, colon length was measured, and colon tissues were collected for pathological analysis. Additionally, the colon contents were analyzed for gut microbiota composition and short-chain fatty acid (SCFA) level, while serum samples were collected to determine inflammatory and oxidative factors. The results indicated that treatment with low, medium, and high doses of LPTAE significantly inhibited weight loss, alleviated rectal bleeding, and reduced colon shortening compared to the DSS group. It also decreased the disease activity index and histopathological activity index scores in the rats. Furthermore, LPTAE reduced the levels of inflammatory cytokines such as IL-1, IL-6, TNF-, and malondialdehyde, while simultaneously increasing the levels of superoxide dismutase and SCFAs, including acetic acid, propionic acid, and butyric acid. 16S rDNA gene sequencing of the gut microbiota revealed that all doses of LPTAE reversed the decrease in both and diversities caused by UC, increased the relative abundance of beneficial bacteria such as , Muribaculaceae, , and , and decreased the levels of harmful bacteria such as , , and . In summary, within the tested doses (100, 150, 250 mg/kg), LPTAE alleviated DSS-induced colitis by modulating the gut microbiota and correcting the metabolic imbalance of SCFAs.
六堡茶以其抗炎、抗氧化和调节肠道微生态平衡的特性而闻名。本研究旨在探讨六堡茶水提取物（LPTAE）对葡聚糖硫酸钠（DSS）诱导的大鼠溃疡性结肠炎（UC）的治疗作用。将大鼠随机分为五组：正常组、DSS组、低剂量六堡茶组（LPTL）、中剂量六堡茶组（LPTM）和高剂量六堡茶组（LPTH）。在整个实验过程中，每天观察并记录大鼠的活动水平、粪便稠度和体重。实验结束后，测量结肠长度，并收集结肠组织进行病理分析。此外，分析结肠内容物的肠道微生物群组成和短链脂肪酸（SCFA）水平，同时收集血清样本以测定炎症和氧化因子。结果表明，与DSS组相比，低、中、高剂量的LPTAE治疗显著抑制体重减轻，减轻直肠出血，并减少结肠缩短。它还降低了大鼠的疾病活动指数和组织病理学活动指数评分。此外，LPTAE降低了白细胞介素-1、白细胞介素-6、肿瘤坏死因子-α和丙二醛等炎症细胞因子的水平，同时提高了超氧化物歧化酶和SCFAs（包括乙酸、丙酸和丁酸）的水平。肠道微生物群的16S rDNA基因测序显示，所有剂量的LPTAE都逆转了UC导致的α和β多样性的降低，增加了有益细菌如拟杆菌属、毛螺菌科、双歧杆菌属和阿克曼菌属的相对丰度，并降低了有害细菌如肠杆菌属、大肠杆菌和肠球菌属的水平。总之，在测试剂量（100、150,250mg/kg）范围内，LPTAE通过调节肠道微生物群和纠正SCFAs的代谢失衡来减轻DSS诱导的结肠炎。
