Peng Yazhou, Zhang Zhi, Wang Min, Shi Xueping, Zhou Yingying, Zhou Yuanhang, Kong Yuan
Key Laboratory of the Three Gorges Reservoir Region's Eco-environment of Ministry of Education, Chongqing University, Chongqing 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-environment of Ministry of Education, Chongqing University, Chongqing 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China.
Ultrason Sonochem. 2020 Dec;69:105254. doi: 10.1016/j.ultsonch.2020.105254. Epub 2020 Jul 12.
Harmful algal blooms pose a potential threat to the safety of drinking water sources. Ultrasound is an effective method for algae removal. However, this method can lead to the release of algal organic matter and the effects and toxic mechanisms of ultrasound on Anabaena are still poorly understood. The destruction mechanism of Anabaena flos-aquae cells under different ultrasonic conditions, the safety of intracellular organic matter (IOM) release to water and the enhanced coagulation efficiency of ultrasound were studied. Results showed that high-frequency ultrasound was effective in breaking down algae cells. After 10 min ultrasonication at 20 kHz, 5 min at 740 kHz and 1 min at 1120 kHz, the algae cells were inactivated and algae growth was halted. Ultrasound radiation can lead to the release of IOM, primarily chlorophyll a and phycocyanin, followed by some tryptophan and humic substances, polysaccharides, and proteins. The sonicated ribosomes were considerably reduced, and the antioxidant system of cells was also damaged to some extent. The coagulation effect of algae cells was substantially improved after ultrasonication. Thus, the safety of algae cell removal could be improved by controlling the changes in physiological structure and IOM release of algae cells by adjusting the ultrasound parameters.
有害藻华对饮用水源安全构成潜在威胁。超声是一种有效的除藻方法。然而，该方法会导致藻有机物的释放，且超声对鱼腥藻的影响及毒性机制仍知之甚少。研究了不同超声条件下水华鱼腥藻细胞的破坏机制、细胞内有机物（IOM）释放到水中的安全性以及超声强化混凝效率。结果表明，高频超声对分解藻细胞有效。在20kHz超声处理10分钟、740kHz处理5分钟和1120kHz处理1分钟后，藻细胞失活，藻生长停止。超声辐射会导致IOM的释放，主要是叶绿素a和藻蓝蛋白，其次是一些色氨酸、腐殖物质、多糖和蛋白质。超声处理后核糖体显著减少，细胞的抗氧化系统也受到一定程度的损害。超声处理后藻细胞的混凝效果显著提高。因此，通过调整超声参数控制藻细胞生理结构和IOM释放的变化，可以提高藻细胞去除的安全性。
