Qiao Jing-Cheng, Zhang Cheng-Lin
Department of Food Engineering, Tianjin Tianshi College, Tianjin, 301700, China.
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science and Technology, Tianjin, 300457, PR China.
Heliyon. 2023 Jul 1;9(7):e17649. doi: 10.1016/j.heliyon.2023.e17649. eCollection 2023 Jul.
Q1, isolated from the eutrophic waters of the Haihe River in Tianjin, possesses remarkable algae dissolving character. We determined the lytic effect of Q1 fermentation broth, and it proved to be pH- and temperature-stable. Then, we investigated the structure of the algicidal compound by high performance liquid chromatography, gas chromatography tandem quadrupole mass spectrometry and fourier transform infrared spectroscopy, and identified as purine-derived CHON. To further understand Q1, we performed genome sequencing and analysis. The genome was 5341610 bp, with 35.31% GC content. Some elements involved in algicidal activity, such as quorum sensing pathway and ABC transporter were predicted. Our results reveal that Q1 can be used for biological control of harmful algal blooms.
从天津海河富营养化水体中分离出的Q1具有显著的溶藻特性。我们测定了Q1发酵液的溶藻效果，结果表明其在pH值和温度方面具有稳定性。然后，我们通过高效液相色谱、气相色谱串联四极杆质谱和傅里叶变换红外光谱研究了杀藻化合物的结构，并鉴定为嘌呤衍生的含碳、氢、氧、氮化合物。为了进一步了解Q1，我们进行了基因组测序和分析。该基因组大小为5341610 bp，GC含量为35.31%。预测了一些与杀藻活性相关的元件，如群体感应途径和ABC转运蛋白。我们的结果表明，Q1可用于有害藻华的生物防治。
