功能化富勒烯对活性氧稳态的影响决定了它们的细胞保护或细胞毒性特性。
Effects of Functionalized Fullerenes on ROS Homeostasis Determine Their Cytoprotective or Cytotoxic Properties.
作者信息
Kostyuk Svetlana V, Proskurnina Elena V, Savinova Ekaterina A, Ershova Elizaveta S, Kraevaya Olga A, Kameneva Larisa V, Umryukhin Pavel E, Dolgikh Olga A, Kutsev Sergey I, Troshin Pavel A, Veiko Natalia N
机构信息
Laboratory of Molecular Biology, Research Centre for Medical Genetics, ul Moskvorechye 1, 115522 Moscow, Russia.
Laboratory of Molecular and Genetic Mechanisms of Adaptation and Stress, Department of Normal Physiology, I.M. Sechenov First Moscow State Medical University (Sechenov University), ul. Mohovaya 11-5, 125007 Moscow, Russia.
出版信息
Nanomaterials (Basel). 2020 Jul 19;10(7):1405. doi: 10.3390/nano10071405.
BACKGROUND
Functionalized fullerenes (FF) can be considered regulators of intracellular reactive oxygen species (ROS) homeostasis; their direct oxidative damage-as well as regulation of oxidant enzymes and signaling pathways-should be considered.
METHODS
Uptake of two water-soluble functionalized C fullerenes with different types of aromatic addends (ethylphenylmalonate and thienylacetate) in human fetal lung fibroblasts, intracellular ROS visualization, superoxide scavenging potential, NOX4 expression, NRF2 expression, oxidative DNA damage, repair genes, cell proliferation and cell cycle were studied.
RESULTS & CONCLUSION: The intracellular effects of ethylphenylmalonate C derivative (FF1) can be explained in terms of upregulated NOX4 activity. The intracellular effects of thienylacetate C derivative (FF2) can be probably resulted from its superoxide scavenging potential and inhibition of lipid peroxidation. FF1 can be considered a NOX4 upregulator and potential cytotoxicant and FF2, as a superoxide scavenger and a potential cytoprotector.
背景
功能化富勒烯（FF）可被视为细胞内活性氧（ROS）稳态的调节剂；应考虑其直接氧化损伤以及对氧化酶和信号通路的调节作用。
方法
研究了两种具有不同类型芳香加成物（乙基苯基丙二酸酯和噻吩基乙酸酯）的水溶性功能化C富勒烯在人胎儿肺成纤维细胞中的摄取、细胞内ROS可视化、超氧化物清除潜力、NOX4表达、NRF2表达、氧化性DNA损伤、修复基因、细胞增殖和细胞周期。
结果与结论
乙基苯基丙二酸酯C衍生物（FF1）的细胞内作用可以通过NOX4活性上调来解释。噻吩基乙酸酯C衍生物（FF2）的细胞内作用可能源于其超氧化物清除潜力和对脂质过氧化的抑制。FF1可被视为NOX4上调剂和潜在的细胞毒性剂，而FF2则可作为超氧化物清除剂和潜在的细胞保护剂。
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