Palmé Anna E, Hagenblad Jenny, Solberg Svein Øivind, Aloisi Karolina, Artemyeva Anna
Nordic Genetic Resource Centre, Smedjevägen 3, SE-230 53 Alnarp, Sweden.
Department of Physics, Chemistry and Biology, Linköping University, SE-581 83 Linköping, Sweden.
Plants (Basel). 2020 Jul 22;9(8):925. doi: 10.3390/plants9080925.
Around the world, there are more than 1500 genebanks storing plant genetic resources to be used in breeding and research. Such resources are essential for future food security, but many genebanks experience backlogs in their conservation work, often combined with limited budgets. Therefore, avoiding duplicate holdings is on the agenda. A process of coordination has started, aiming at sharing the responsibility of maintaining the unique accessions while allowing access according to the international treaty for plant genetic resources. Identifying duplicate holdings based on passport data has been one component of this. In the past, and especially in vegetables, different selections within the same varieties were common and the naming practices of cultivars/selections were flexible. Here, we examined 10 accession pairs/groups of cabbage ( var. ) with similar names maintained in the Russian and Nordic genebanks. The accessions were analyzed for 11 morphological traits and with a SNP (Single Nucleotide Polymorphism) array developed for . Both proved to be useful tools for understanding the genetic structure among the accessions and for identifying duplicates, and a subset of 500 SNP markers are suggested for future genetic characterization. Within five out of 10 pairs/groups, we detected clear genetic differences among the accessions, and three of these were confirmed by significant differences in one or several morphological traits. In one case, a white cabbage and a red cabbage had similar accession names. The study highlights the necessity to be careful when identifying duplicate accessions based solely on the name, especially in older cross-pollinated species such as cabbage.
在全球范围内，有超过1500个基因库储存着用于育种和研究的植物遗传资源。这些资源对未来的粮食安全至关重要，但许多基因库在其保护工作中存在积压，且往往预算有限。因此，避免重复保存已提上日程。一个协调过程已经启动，旨在分担维护独特种质的责任，同时根据植物遗传资源国际条约允许获取。基于护照数据识别重复保存是其中的一个组成部分。过去，尤其是在蔬菜方面，同一品种内的不同选系很常见，品种/选系的命名做法也很灵活。在此，我们研究了俄罗斯和北欧基因库中保存的10对/组名称相似的甘蓝（变种）种质。对这些种质分析了11个形态性状，并使用了为[具体物种]开发的单核苷酸多态性（SNP）芯片。两者都被证明是理解种质间遗传结构和识别重复种质的有用工具，建议使用500个SNP标记的子集用于未来的遗传特征分析。在10对/组中的5对/组内，我们检测到种质间存在明显的遗传差异，其中3对/组通过一个或几个形态性状的显著差异得到证实。在一个案例中，一个结球甘蓝和一个紫甘蓝有着相似的种质名称。该研究强调了仅根据名称识别重复种质时要谨慎的必要性，尤其是在像甘蓝这样的古老异花授粉物种中。
