Lai Xiaoyi, Xu Jie, Ma Haihao, Liu Zheming, Zheng Wei, Liu Jia, Zhu Hang, Zhou Yong, Zhou Xiaomao
Long Ping Branch, Graduate School of Hunan University, Changsha 410125, China.
Institute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Insects. 2020 Jul 22;11(8):461. doi: 10.3390/insects11080461.
In insects, inward-rectifying potassium (Kir) channels regulate vital physiological functions, such as feeding behavior, silk secretion, renal excretion, and immune function. Therefore, they offer promising potential as targets for insecticides. Three types of Kir subunits have been identified in Diptera and Hemiptera, but the Kir subunits of Lepidoptera still remain unclear. This study identified five Kir subunit genes (, , , , and ) in the transcriptome of . Phylogenetic analysis identified , , , and as orthologous genes of - in other insects. Interestingly, may be encoding a new class of Kir subunit in Lepidoptera that has not been reported to date. To identify further Kir channel subunits of , the gene expression profiles of five genes were studied by quantitative real-time PCR. These genes are expressed throughout the development of . and were highly expressed in thoraxes and legs, while (3A and 3B) and had high expression levels in the midgut and Malpighian tubules. This study identified the composition and distribution of Kir subunits in for the first time, and provides useful information for the further study of Kir channel subunits in Lepidoptera.
在昆虫中，内向整流钾（Kir）通道调节重要的生理功能，如取食行为、吐丝、排泄和免疫功能。因此，它们作为杀虫剂的靶标具有广阔的潜力。在双翅目和半翅目中已鉴定出三种类型的Kir亚基，但鳞翅目昆虫的Kir亚基仍不清楚。本研究在[某种昆虫名称]的转录组中鉴定出五个Kir亚基基因（[基因名称1]、[基因名称2]、[基因名称3]、[基因名称4]和[基因名称5]）。系统发育分析确定[基因名称1]、[基因名称2]、[基因名称3]和[基因名称4]是其他昆虫中[相关基因名称]的直系同源基因。有趣的是，[基因名称5]可能编码鳞翅目中一类迄今尚未报道的新型Kir亚基。为了进一步鉴定[某种昆虫名称]的Kir通道亚基，通过定量实时PCR研究了五个[基因名称]基因的表达谱。这五个[基因名称]基因在[某种昆虫名称]的整个发育过程中均有表达。[基因名称1]和[基因名称2]在胸部和腿部高表达，而[基因名称3]（3A和3B）和[基因名称4]在中肠和马氏管中表达水平较高。本研究首次确定了[某种昆虫名称]中Kir亚基的组成和分布，为进一步研究鳞翅目昆虫的Kir通道亚基提供了有用信息。
