Liu Jia, Liu Zheming, Ma Haihao, Yu Yaying, Zhang Chengjia, Zheng Wei, Man Yilong, Zhu Hang, Zhou Yong, Chen Xi, Zhou Xiaomao, Zeng Aiping
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
Institute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Insects. 2022 Mar 30;13(4):340. doi: 10.3390/insects13040340.
Trehalase regulates energy metabolism in insects by converting trehalose into two glucose molecules. High amounts of trehalase are critical for insect flight and larval stress resistance. However, whether trehalase participates in the development of pesticide resistance remains unclear. In this study, we explored this phenomenon and the mechanism that underlies the regulation of transcription. We found that overexpression of 1 and 2 induced (Bt) resistance in . The promoter sequences of 1 and 2 were also cloned and identified. The dual-luciferase reporter system and RNA interference technology revealed that the expression of 1 and 2 genes is possibly regulated by the CCAAT enhancer-binding protein (C/EBPα). A yeast one-hybrid experiment confirmed the interaction between C/EBPα and the 2 promoter. The findings of this study suggest that C/EBPα mediates the adaptability of to adverse environmental stressors by regulating the expression of trehalase.
海藻糖酶通过将海藻糖转化为两个葡萄糖分子来调节昆虫的能量代谢。大量的海藻糖酶对昆虫飞行和幼虫抗逆性至关重要。然而，海藻糖酶是否参与抗药性的形成仍不清楚。在本研究中，我们探究了这一现象以及转录调控的潜在机制。我们发现1和2的过表达诱导了[昆虫名称]对苏云金芽孢杆菌（Bt）的抗性。还克隆并鉴定了1和2的启动子序列。双荧光素酶报告系统和RNA干扰技术表明，1和2基因的表达可能受CCAAT增强子结合蛋白（C/EBPα）调控。酵母单杂交实验证实了C/EBPα与2启动子之间的相互作用。本研究结果表明，C/EBPα通过调节海藻糖酶的表达介导了[昆虫名称]对不利环境应激源的适应性。
