Hiruma Sumiyo, Ishihara Masayuki, Nakamura Shingo, Sato Yoko, Asahina Haruka, Fukuda Koichi, Takayama Tomohiro, Murakami Kaoru, Yokoe Hidetaka
Division of Biomedical Engineering, Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Division of Statistical Analysis, Research Support Center, Shizuoka General Hospital, 4-27-1 Kita-ando, Aoi-ku, Shizuoka 420-8527, Japan.
Foods. 2020 Jul 21;9(7):964. doi: 10.3390/foods9070964.
Recently, there has been an increase in the number of food poisoning cases associated with histamine in food, mainly in relation to histamine in fish. Here, we investigated methods to decrease histamine levels in Japanese pilchard, Japanese horse mackerel, and chub Mackerel, stored at 10 °C using various concentrations of heated scallop bioshell calcium oxide (BiSCaO) suspension, dispersion (BiSCaO + NaHPO), colloidal dispersion (BiSCaO + NapolyPO), scallop shell powder (SSP) Ca(OH) in pure water (PW) or saline, and BiSCaO water. BiSCaO in a high alkaline pH solution chemically decomposes histamine poorly, but the partial flocculation/precipitation of histamine was observed with 1 and 0.2 wt.% BiSCaO dispersion and BiSCaO colloidal dispersion, respectively. Cleaning fish samples with BiSCaO suspension, dispersion, colloidal dispersion, or BiSCaO water remarkably reduced histamine levels and normal bacterial flora (coliform bacteria (CF) and total viable bacterial cells (TC)) after storage for four days at 10 °C, while much higher histamine levels were observed after cleaning with saline. These results suggest that cleaning fish with BiSCaO dispersion, colloidal dispersion, or BiSCaO water can significantly reduce histamine levels through their bactericidal activity against histamine-producing bacteria.
最近，与食物中组胺相关的食物中毒病例数量有所增加，主要与鱼类中的组胺有关。在此，我们研究了使用不同浓度的加热扇贝生物壳氧化钙（BiSCaO）悬浮液、分散液（BiSCaO + NaHPO）、胶态分散液（BiSCaO + NapolyPO）、纯水中的扇贝壳粉（SSP）Ca(OH)或生理盐水以及BiSCaO水，来降低在10°C下储存的日本沙丁鱼、日本竹荚鱼和鲐鱼中组胺水平的方法。在高碱性pH溶液中的BiSCaO对组胺的化学分解效果不佳，但分别在1 wt.%和0.2 wt.%的BiSCaO分散液和BiSCaO胶态分散液中观察到了组胺的部分絮凝/沉淀现象。用BiSCaO悬浮液、分散液、胶态分散液或BiSCaO水清洗鱼样品，在10°C下储存四天后，组胺水平和正常细菌菌群（大肠菌群（CF）和总活菌数（TC））显著降低，而用生理盐水清洗后观察到的组胺水平则高得多。这些结果表明，用BiSCaO分散液、胶态分散液或BiSCaO水清洗鱼可以通过其对产组胺细菌的杀菌活性显著降低组胺水平。
