Integrated Metabolomics and Transcriptomics Analyses Reveal the Molecular Mechanisms Underlying the Accumulation of Anthocyanins and Other Flavonoids in Cowpea Pod ( L.).

As an important vegetable crop of the legume family, cowpea ( L.) is grown widely for its tender pod with good taste and nutrition. The purple cowpea pods attract more attention mainly for the eye-catching color and health-promoting ingredients. Initially, large quantities of two major anthocyanins (delphinidin 3--glucoside and cyanidin 3--glucoside) and nine kinds of flavonoids (most are quercetin-based flavonol glycosides) were separated and identified from purple cowpea pod by ultra-high performance liquid chromatography coupled with quadrupole Orbitrap high-resolution mass spectrometry. To study them systematically, two representative cowpea cultivars with a drastic difference in anthocyanin accumulation were further analyzed by the integration of metabolomics and transcriptomics. A total of 56 differentially accumulated metabolites and 4142 differentially expressed genes were identified, respectively. On the basis of the comprehensive analysis of multiomic data, it was shown that VuMYB90-1, VuMYB90-2, VuMYB90-3, VuCPC, VuMYB4, and endogenous bHLH and WD40 proteins coordinately control anthocyanin and flavonoid accumulation via transcriptional regulation of structural genes in purple cowpea pod.