Guangdong Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangzhou510316, China; Guangdong Engineering Research Center for Sugar Technology, Guangzhou510316, China.
Guangdong Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangzhou510316, China; Guangdong Engineering Research Center for Sugar Technology, Guangzhou510316, China.
J Chromatogr A. 2020 Aug 16;1625:461305. doi: 10.1016/j.chroma.2020.461305. Epub 2020 Jun 3.
A novel kind of magnetic porous carbon nano-fibers (FeO@P-CNFs) materials was successfully prepared and used as an adsorbent. Based on the above-mentioned adsorbent, a simple and effective magnetic disperse solid-phase extraction (MSPE) method was developed and first utilized to the enrichment and purification of five Sudan dyes (including Sudan I, Sudan II, Sudan III, Sudan IV, and Sudan Red 7B) in foodstuffs for the first time. High-performance liquid chromatography was used to determine the content of the Sudan dyes. The parameters affecting the extraction performance were studied and optimized, including the amount of the adsorbent and inorganic salt, type and the volume of the eluent, pH of the sample solution and extraction time. Under the optimized experimental conditions, the results show that the proposed method has a good linear relationship (r≥ 0.9993). The limits of detection range from 0.88 μg L to 1.27 μg L. The recoveries range from 86.6% to 99.7% with the relative standard deviations ranging from 0.6% to 7.9% in the methodology validation. The above-mentioned results indicate that the proposed method is a sensitive and reliable procedure with good reproducibility for the detection of Sudan dyes residues in foodstuffs.
一种新型磁性多孔碳纳米纤维（FeO@P-CNFs）材料被成功制备并用作吸附剂。基于上述吸附剂，开发了一种简单有效的磁性分散固相萃取（MSPE）方法，并首次用于食品中五种苏丹染料（苏丹红 I、苏丹红 II、苏丹红 III、苏丹红 IV 和苏丹红 7B）的富集和净化。高效液相色谱法用于测定苏丹染料的含量。研究并优化了影响萃取性能的参数，包括吸附剂和无机盐的用量、洗脱剂的类型和体积、样品溶液的 pH 值和萃取时间。在优化的实验条件下，结果表明，该方法具有良好的线性关系（r≥0.9993）。检测限范围为 0.88μg/L 至 1.27μg/L。在方法验证中，回收率范围为 86.6%至 99.7%，相对标准偏差范围为 0.6%至 7.9%。上述结果表明，该方法是一种灵敏、可靠的方法，具有良好的重现性，可用于检测食品中苏丹染料残留。
