National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan; Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo 113-0033, Japan.
National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
J Chromatogr A. 2020 Aug 16;1625:461306. doi: 10.1016/j.chroma.2020.461306. Epub 2020 Jun 6.
A pentafluorobenzoylation (PFBz)-liquid chromatography-tandem mass spectrometry method was developed for qualitative and quantitative analysis of ethanolamines (EAs, nitrogen mustard degradation products). With this method, highly hydrophilic EAs can be sufficiently analyzed with a commonly used reversed phase column (retention times: (PFBz)-methyl diethanolamine, 9.1 min; (PFBz)-ethyl diethanolamine, 9.8 min; and (PFBz)-triethanolamine, 17.6 min). The applicability of the method for real samples was investigated via recovery tests. Methyl diethanolamine and ethyl diethanolamine were detected at concentrations as low as 1 ng/mL in serum and 10 ng/mL in urine, and quantified within the range of 1-1000 ng/mL and 10-1000 ng/mL, respectively.
建立了一种五氟苯甲酰化（PFBz）-液相色谱-串联质谱法，用于定性和定量分析乙醇胺（EAs，氮芥降解产物）。使用该方法，通常使用的反相柱（保留时间：（PFBz）-甲基二乙醇胺，9.1 分钟；（PFBz）-乙基二乙醇胺，9.8 分钟；和（PFBz）-三乙醇胺，17.6 分钟）可充分分析高亲水性的 EAs。通过回收试验研究了该方法对实际样品的适用性。在血清中，甲代二乙醇胺和乙代二乙醇胺的检测浓度低至 1ng/mL，在尿液中检测浓度低至 10ng/mL，且在 1-1000ng/mL 和 10-1000ng/mL 范围内可定量。
