Unequal rewarding of three metabolizable sugars - sucrose, fructose and glucose - in olfactory learning and memory in .

Learning and memory are the most characterized advanced neurological activities of insects, which can associate information with food. Our previous studies on have shown that this fly can learn to evaluate the nutritional value of sugar rewards, although whether all metabolizable sugars are equally rewarding to flies is still unclear. To address this question, we used three sweet and metabolizable sugars - sucrose, fructose and glucose - as rewards for conditioning. The flies showed differences in learning and memory in response to the three sugar rewards. The level of learning performance in sucrose-rewarded flies was higher than that in fructose-rewarded and glucose-rewarded flies, and, strikingly, only sucrose and glucose stimulation led to the formation of robust 24-h memory. Furthermore, the unequal rewarding of three sugars was observed in two distinct processes of memory formation: preingestive and postingestive processes. When flies received the positive tastes (preingestive signal) by touching their tarsi and proboscis (mouthparts) to three sugars, they showed differences in learning for the three sugar rewards. The formation of a robust 24-h memory was dependent on the postingestive signal triggered by feeding on a sugar. A deficit of 24-h memory was observed only in fructose-feeding flies no matter what sugar was used to stimulate the tarsi. Taken together, our results suggest that three sweet and metabolizable sugars unequally rewarded , which might be a strategy for flies to discriminate the nature of sugars.