School of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, 116024, No. 2, Linggong Rd, Dalian, Liaoning Province, China.
School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, 116024, No. 2, Linggong Rd, Dalian, Liaoning Province, China.
Biomech Model Mechanobiol. 2021 Feb;20(1):55-67. doi: 10.1007/s10237-020-01368-7. Epub 2020 Jul 24.
Revealing the mechanisms underlying the intracellular calcium responses in vascular endothelial cells (VECs) induced by mechanical stimuli contributes to a better understanding for vascular diseases, including hypertension, atherosclerosis, and aneurysm. Combining with experimental measurement and Computational Fluid Dynamics simulation, we developed a mechanobiological model to investigate the intracellular [Ca] response in a single VEC being squeezed through narrow microfluidic channel. The time-dependent cellular surface tension dynamics was quantified throughout the squeezing process. In our model, the various Ca signaling pathways activated by mechanical stimulation is fully considered. The simulation results of our model exhibited well agreement with our experimental results. By using the model, we theoretically explored the mechanism of the two-peak intracellular [Ca] response in single VEC being squeezed through narrow channel and made some testable predictions for guiding experiment in the future.
揭示机械刺激诱导血管内皮细胞（VEC）内钙离子反应的机制有助于更好地理解血管疾病，包括高血压、动脉粥样硬化和动脉瘤。我们结合实验测量和计算流体动力学模拟，开发了一种机械生物学模型来研究单个 VEC 在通过狭窄微流道时的细胞内 [Ca] 反应。在挤压过程中定量了随时间变化的细胞表面张力动力学。在我们的模型中，充分考虑了机械刺激激活的各种 Ca 信号通路。我们模型的模拟结果与实验结果吻合较好。通过使用该模型，我们从理论上探讨了单 VEC 通过狭窄通道挤压时双峰细胞内 [Ca] 反应的机制，并对未来的实验提出了一些可测试的预测。
