, Jinzhong, Shanxi, People's Republic of China.
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, People's Republic of China.
Biol Trace Elem Res. 2021 Apr;199(4):1493-1500. doi: 10.1007/s12011-020-02264-y. Epub 2020 Jul 24.
To investigate the mechanism of fluoride-induced splenic toxicity, 0, 25, 50, and 100 mg/L sodium fluoride (NaF) were administered in male mice via drinking water for 90 days. After NaF treatment, the histological structure of the spleen, the proportion of helper T 1 cell (Th1) and helper T 2 cell (Th2), and the relative expression levels of cytokines and T-bet and GATA3 were analyzed. The results showed that 50 and 100 mg/L NaF consumption can change the normal structure of mouse spleen and the proportion of Th1/Th2 cells. It also decreased the mRNA expression levels of IL-2, INF-γ, and TGF-β, but increased the levels of IL-4, IL-6, and IL-10. Importantly, fluoride increased the protein expression of GATA3 but decreased the expression of T-bet. Our findings indicate that superfluous fluoride intake damages the balance of Th1/Th2 cells by changing the levels of T-bet and GATA3 in the spleen, and further changes the expression of Th1/Th2 cell-related cytokines in the spleen microenvironment, eventually leading to spleen injury.
为了研究氟化物诱导的脾毒性机制，雄性小鼠通过饮用水摄入 0、25、50 和 100mg/L 的氟化钠（NaF），为期 90 天。NaF 处理后，分析了脾脏的组织学结构、辅助性 T 细胞 1 型（Th1）和辅助性 T 细胞 2 型（Th2）的比例，以及细胞因子和 T-bet、GATA3 的相对表达水平。结果表明，50 和 100mg/L 的 NaF 摄入可改变小鼠脾脏的正常结构和 Th1/Th2 细胞的比例。还降低了 IL-2、INF-γ 和 TGF-β 的 mRNA 表达水平，但增加了 IL-4、IL-6 和 IL-10 的水平。重要的是，氟化物增加了 GATA3 的蛋白表达，但降低了 T-bet 的表达。我们的研究结果表明，过量的氟摄入通过改变脾脏中 T-bet 和 GATA3 的水平破坏了 Th1/Th2 细胞的平衡，进而改变了脾脏微环境中 Th1/Th2 细胞相关细胞因子的表达，最终导致脾脏损伤。
