健康献血者单核细胞分布宽度（MDW）的参考区间。
Reference interval of monocyte distribution width (MDW) in healthy blood donors.
作者信息
Agnello Luisa, Lo Sasso Bruna, Bivona Giulia, Gambino Caterina Maria, Giglio Rosaria Vincenza, Iacolino Giorgia, Iacona Alessandro, Mancuso Silvia, Ciaccio Anna Maria, Vidali Matteo, Ciaccio Marcello
机构信息
Institute of Clinical Biochemistry, Clinical Molecular Medicine and Laboratory Medicine, Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, Palermo, Italy.
Institute of Clinical Biochemistry, Clinical Molecular Medicine and Laboratory Medicine, Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, Palermo, Italy; Department of Laboratory Medicine, University Hospital "P. Giaccone", Palermo, Italy.
出版信息
Clin Chim Acta. 2020 Nov;510:272-277. doi: 10.1016/j.cca.2020.07.036. Epub 2020 Jul 22.
BACKGROUND
The aim of the study was to accurately establish the reference interval (RI) of monocyte distribution width (MDW) in healthy blood donors by the direct method using different statistical approaches.
METHODS
MDW was measured in 486 subjects. RI of MDW was calculated by the non-parametric method, the robust method and, the Harrell-Davis bootstrap method and using different tests to identify potential outliers (Dixon-Reed and Tukey).
RESULTS
Lower and upper reference limits of the RI calculated by the non-parametric method were, 16.22 (90%CI 15.78-16.47) - 23.15 (90%CI 22.80-24.10) (without outlier removal), and 16.44 (90%CI 16.21-16.67) - 22.99 (90%CI 22.33-23.22) (after outlier removal). The RIs based on the robust method were, respectively, 16.29-22.98 (without) and 16.50-22.67 (with outlier removal). Finally, the RIs calculated by the Harrell-Davis bootstrap method, without or after outlier removal, were 16.19-23.24 and 16.43-22.93. Thus, the RIs obtained by the three calculation methods were very similar. Additionally, no RI partition was done since no significant gender or age association was found.
CONCLUSIONS
Our results support the use of a unique RI of MDW, independently of sex and age.
背景
本研究的目的是通过使用不同统计方法的直接法准确建立健康献血者单核细胞分布宽度（MDW）的参考区间（RI）。
方法
对486名受试者进行MDW测量。通过非参数法、稳健法和哈雷尔-戴维斯自助法计算MDW的RI，并使用不同检验识别潜在离群值（狄克逊-里德检验和 Tukey 检验）。
结果
非参数法计算的RI下限和上限分别为16.22（90%CI 15.78 - 16.47） - 23.15（90%CI 22.80 - 24.10）（未去除离群值），以及16.44（90%CI 16.21 - 16.67） - 22.99（90%CI 22.33 - 23.22）（去除离群值后）。基于稳健法的RI分别为16.29 - 22.98（未去除）和16.50 - 22.67（去除离群值）。最后，哈雷尔-戴维斯自助法计算的RI，无论是否去除离群值，分别为16.19 - 23.24和16.43 - 22.93。因此，三种计算方法得到的RI非常相似。此外，由于未发现显著的性别或年龄关联，未进行RI划分。
结论
我们的结果支持使用单一的MDW参考区间，而不考虑性别和年龄。
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