鸡对肠炎沙门氏菌血清型感染反应中免疫和能量代谢相关基因的相关表达。
The correlated expression of immune and energy metabolism related genes in the response to Salmonella enterica serovar Enteritidis inoculation in chicken.
机构信息
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 271018, Taian, China.
Present Address: Current affiliation: Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, Beijing, China.
出版信息
BMC Vet Res. 2020 Jul 25;16(1):257. doi: 10.1186/s12917-020-02474-5.
BACKGROUND
Salmonella enterica serovar Enteritidis (SE) is one of the food-borne pathogenic bacteria, which affects poultry production and poses severe threat to human health. The correlation of immune system and metabolism in chicken after SE inoculation is important but not clear. In the current study, we identified the expression of immune and energy metabolism related genes using quantitative PCR to evaluate the correlation between immune system and energy metabolism against SE inoculation in Jining Bairi chicken.
RESULTS
ATP5G1, ATP5G3 and ND2 were significantly up-regulated at 1 dpi (day post inoculation), and ATP5E, ATP5G1, ATP5G3 were significantly down-regulated at 7 dpi (P < 0.05). IL-8 and IL-1β were significantly down-regulated at 1 dpi, IL-8 and IL-18 were significantly down-regulated at 3 dpi, IL-8 and BCL10 were significantly up-regulated at 7 dpi (P < 0.05).
CONCLUSIONS
These findings indicate that the correlation between immune and energy metabolism related genes gradually change with time points post SE inoculation, from one homeostasis to an opposite homeostasis with 3 dpi as a turning point. These results will pave the foundation for the relationship between immune system and energy metabolism in the response to SE inoculation in chicken.
背景
肠炎沙门氏菌（SE）是食源性致病菌之一，影响家禽生产，对人类健康构成严重威胁。SE 接种后鸡的免疫系统和代谢之间的相关性很重要，但尚不清楚。在本研究中，我们使用定量 PCR 鉴定免疫和能量代谢相关基因的表达，以评估济宁百日鸡对 SE 接种的免疫系统和能量代谢之间的相关性。
结果
在 1dpi（接种后第 1 天）时，ATP5G1、ATP5G3 和 ND2 显著上调，在 7dpi（接种后第 7 天）时，ATP5E、ATP5G1 和 ATP5G3 显著下调（P<0.05）。在 1dpi 时，IL-8 和 IL-1β 显著下调，在 3dpi 时，IL-8 和 IL-18 显著下调，在 7dpi 时，IL-8 和 BCL10 显著上调（P<0.05）。
结论
这些发现表明，SE 接种后免疫和能量代谢相关基因之间的相关性随着时间点的变化而逐渐变化，从一个平衡状态到以 3dpi 为转折点的相反平衡状态。这些结果将为鸡对 SE 接种的免疫系统和能量代谢之间的关系奠定基础。
Zotero 插件