Liu Liying, Lin Lili, Zheng Linna, Tang Hui, Fan Xinzhong, Xue Nianguo, Li Min, Liu Min, Li Xianyao
2College of Life Science, Shandong Agricultural University, Tai'an, 271000 Shandong China.
1College of Animal Science and Technology, Shandong Agricultural University, Tai'an, 271000 Shandong China.
Gut Pathog. 2018 Aug 3;10:34. doi: 10.1186/s13099-018-0261-x. eCollection 2018.
, serovar Enteritidis ( Enteritidis), an important zoonotic foodborne pathogen, can affect the microbiota of the chicken intestine and cause many enteric diseases, such as acute gastroenteritis. The gut microbiota contributes to the development and function of the host immune system and competes with pathogenic microbes. The interaction between Enteritidis and the host cecal microbiota is still not fully understood. We investigated the microbiome composition in both treated and control groups through 16S ribosomal RNA (rRNA) gene sequencing at 1, 3, 7, 14, 21, 28, and 35 days post- Enteritidis inoculation (dpi) in the current study.
Chao1 richness and Shannon diversity significantly increased with chicken development in both the treated and control groups ( < 0.05). The Chao1 index was significantly lower in the treated group than that in the control group at 14 dpi ( < 0.05). Phyla and were most dominant at 1 and 3 dpi. Enteritidis inoculation influenced cecal microbiota mainly at 7 and 14 dpi. Enteritidis inoculation significantly altered the relative abundance of 18 genera at different time points ( < 0.05) with relative abundance significantly changed after 14 dpi. The abundance of those genera changed dramatically between 28 and 35 dpi in the treated group compared to control group. Positive correlations existed between and and between and following . Enteritidis inoculation.
Our results indicated that both development and Enteritidis have effect on chicken cecal microbiota profiles. Enteritidis inoculation in young chicks influences the cecal microbiota mainly at 7 and 14 dpi. The cecal microbiota exhibited immunity to Enteritidis inoculation at 28 dpi. These findings will provide basic knowledge of the role that chicken cecal microbiota play in response to Enteritidis inoculation.
肠炎沙门氏菌血清型肠炎亚种（肠炎沙门氏菌）是一种重要的人畜共患食源性病原体，可影响鸡肠道微生物群并引发多种肠道疾病，如急性肠胃炎。肠道微生物群有助于宿主免疫系统的发育和功能，并与致病微生物竞争。肠炎沙门氏菌与宿主盲肠微生物群之间的相互作用仍未完全了解。在本研究中，我们通过16S核糖体RNA（rRNA）基因测序，在肠炎沙门氏菌接种后1、3、7、14、21、28和35天（dpi）对治疗组和对照组的微生物组组成进行了调查。
在治疗组和对照组中，Chao1丰富度和香农多样性均随鸡的发育而显著增加（P<0.05）。在14 dpi时，治疗组的Chao1指数显著低于对照组（P<0.05）。在1和3 dpi时，厚壁菌门和拟杆菌门最为占主导地位。肠炎沙门氏菌接种主要在7和14 dpi时影响盲肠微生物群。肠炎沙门氏菌接种在不同时间点显著改变了18个属（P<0.05）的相对丰度，在14 dpi后相对丰度发生显著变化。与对照组相比，治疗组中这些属的丰度在28至35 dpi之间变化显著。肠炎沙门氏菌接种后，厚壁菌门与拟杆菌门之间以及双歧杆菌属与乳酸菌属之间存在正相关。
我们的结果表明，发育和肠炎沙门氏菌均对鸡盲肠微生物群谱有影响。幼雏接种肠炎沙门氏菌主要在7和14 dpi时影响盲肠微生物群。盲肠微生物群在28 dpi时对肠炎沙门氏菌接种表现出免疫力。这些发现将为鸡盲肠微生物群在应对肠炎沙门氏菌接种中所起的作用提供基础知识。
