Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India.
Biol Trace Elem Res. 2021 May;199(5):1707-1714. doi: 10.1007/s12011-020-02298-2. Epub 2020 Jul 25.
Occupational exposure to lead (Pb) remains a significant concern for worker's health working in different factories. There are many discrepancies among the results regarding the studies of genotoxicity of Pb. The present study aimed to evaluate DNA damage and expressions of DNA repair genes (OGG1, XRCC1, and XPD) in occupationally Pb-exposed workers of Jodhpur, India. The study consisted of 100 occupationally Pb-exposed workers and 100 controls (non-exposed) with no history of occupational exposure. Pb levels were determined by atomic absorption spectrophotometry, serum 8-hydroxy-2-deoxyguanosine (8-OHdG) concentrations were measured by ELISA, and expressions of DNA repair genes (OGG1, XRCC1, and XPD) were estimated by RT-PCR. The results indicated significantly higher levels of Pb in the exposed group as compared with the non-exposed group (p < 0.0001). Serum 8-OHdG concentrations were significantly higher (p < 0.0083), and all three DNA repair genes were significantly downregulated (fold change: OGG1, 0.188; XRCC1, 0.125; XPD, 0.133) in the Pb-exposed group as compared with the non-exposed. In conclusion, the study findings suggest that Pb exposure is associated with increased DNA damage and reduced DNA repair capacity, which may lead to serious health issues in occupationally Pb-exposed workers.
职业性铅暴露（Pb）仍然是从事不同工厂工作的工人健康的一个重大关注点。关于 Pb 的遗传毒性研究结果存在许多差异。本研究旨在评估印度焦特布尔职业性 Pb 暴露工人的 DNA 损伤和 DNA 修复基因（OGG1、XRCC1 和 XPD）的表达。该研究包括 100 名职业性 Pb 暴露工人和 100 名对照（非暴露）工人，他们没有职业暴露史。通过原子吸收分光光度法测定 Pb 水平，通过 ELISA 测定血清 8-羟基-2-脱氧鸟苷（8-OHdG）浓度，通过 RT-PCR 估计 DNA 修复基因（OGG1、XRCC1 和 XPD）的表达。结果表明，暴露组的 Pb 水平明显高于非暴露组（p<0.0001）。血清 8-OHdG 浓度明显升高（p<0.0083），与非暴露组相比，Pb 暴露组所有三种 DNA 修复基因的表达均明显下调（倍数变化：OGG1，0.188；XRCC1，0.125；XPD，0.133）。总之，研究结果表明，Pb 暴露与 DNA 损伤增加和 DNA 修复能力降低有关，这可能导致职业性 Pb 暴露工人出现严重的健康问题。
