构象分析探究剪接过程中 II 型内含子的构象动态。
SHAPE Profiling to Probe Group II Intron Conformational Dynamics During Splicing.
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
出版信息
Methods Mol Biol. 2021;2167:171-182. doi: 10.1007/978-1-0716-0716-9_10.
Abstract
Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) is a widely used technique for studying the structure and function of RNA molecules. It characterizes the flexibility of single nucleotides in the context of the local RNA structure. Here we describe the application of SHAPE-MaP (mutational profiling) to study different conformational states of the group II intron during the self-splicing reaction.
摘要
选择性 2'-羟乙酰化分析引物延伸（SHAPE）是一种广泛用于研究 RNA 分子结构和功能的技术。它描述了在局部 RNA 结构背景下单个核苷酸的灵活性。在这里，我们描述了 SHAPE-MaP（突变分析）在研究自我剪接反应过程中 II 类内含子不同构象状态中的应用。
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