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用于韧皮部取食昆虫食物摄入研究的实用简易系统。

Practical and Simple Systems for Food Intake Studies on Phloem-Sucking Insects.

作者信息

Alves G R, Diniz A J F, Vieira J M, Silveira C H, Parra J R P

机构信息

Dept of Entomology and Acarology, Luiz de Queiroz College of Agriculture, Univ of São Paulo, Piracicaba, SP, Brasil.

Koppert Biological Systems, Piracicaba, SP, Brasil.

出版信息

Neotrop Entomol. 2021 Feb;50(1):156-160. doi: 10.1007/s13744-020-00803-9. Epub 2020 Jul 25.

DOI:10.1007/s13744-020-00803-9
PMID:32712943
Abstract

Studies of insect feeding behavior are useful in different areas of entomology such as plant resistance, biology, and insecticide efficacy. For chewing insects, this kind of technique is well established, but for sap-sucking insects, especially tiny ones such as whiteflies, aphids, and psyllids, these tests can be laborious. Manipulation is difficult and can damage the plant, affecting the results. We describe here three types of cages for tests with small insects, one for seedlings in pots, a second for larger plants or plants in the field, and a third for caging insects on part of a leaf. These cages have been useful for different types of studies in addition to feeding behavior and can facilitate research with small phytophagous sucking insects.

摘要

昆虫取食行为的研究在昆虫学的不同领域都很有用,如植物抗性、生物学和杀虫剂功效等。对于咀嚼式昆虫,这种技术已很成熟,但对于吸食汁液的昆虫,尤其是粉虱、蚜虫和木虱等微小昆虫,这些测试可能会很费力。操作困难且可能会损害植物,从而影响结果。我们在此描述三种用于小型昆虫测试的笼子,一种用于盆栽幼苗,第二种用于较大的植物或田间植物,第三种用于将昆虫圈养在叶片的一部分上。除了取食行为外,这些笼子还对不同类型的研究有用,并且可以促进对小型植食性吸食昆虫的研究。

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本文引用的文献

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Antifeedant and sublethal effects of imidacloprid on Asian citrus psyllid, Diaphorina citri.吡虫啉对亚洲柑橘木虱(Diaphorina citri)的拒食及亚致死效应
Pest Manag Sci. 2009 Aug;65(8):870-7. doi: 10.1002/ps.1767.
2
Applications of the ninhydrin reaction for analysis of amino acids, peptides, and proteins to agricultural and biomedical sciences.茚三酮反应在氨基酸、肽和蛋白质分析中的应用于农业和生物医学科学领域。
J Agric Food Chem. 2004 Feb 11;52(3):385-406. doi: 10.1021/jf030490p.
3
Standardisation of the nitro-blue tetrazolium test and factors affecting its clinical application.
硝基蓝四氮唑试验的标准化及其临床应用的影响因素。
S Afr Med J. 1974 Feb 9;48(6):209-12.