Institute of Insect Science, Zhejiang University, Hangzhou, China.
Department of Pathology of Sir Run Run Shaw Hospital, and Center of Cryo-Electron Microscopy, School of Medicine, Zhejiang University, Hangzhou, China.
Elife. 2021 Feb 23;10:e62875. doi: 10.7554/eLife.62875.
Using serial block-face scanning electron microscopy, we report on the internal 3D structures of the brown planthopper, (Hemiptera: Delphacidae) at nanometer resolution for the first time. Within the reconstructed organs and tissues, we found many novel and fascinating internal structures in the planthopper such as naturally occurring three four-way rings connecting adjacent spiracles to facilitate efficient gas exchange, and fungal endosymbionts in a single huge insect cell occupying 22% of the abdomen volume to enable the insect to live on plant sap. To understand the muscle and stylet movement during phloem sap-sucking, the cephalic skeleton and muscles were reconstructed in feeding nymphs. The results revealed an unexpected contraction of the protractors of the stylets and suggested a novel feeding model for the phloem sap-sucking.
利用连续块面扫描电子显微镜，我们首次以纳米分辨率报告了褐飞虱（半翅目：飞虱科）的内部 3D 结构。在重建的器官和组织中，我们在飞虱中发现了许多新颖而迷人的内部结构，例如自然形成的三个四通环连接相邻的呼吸孔，以促进高效气体交换，以及单个巨大昆虫细胞中的真菌内共生体，占据腹部体积的 22%，使昆虫能够以植物汁液为食。为了了解在韧皮部汁液吸食过程中的肌肉和口针运动，我们在取食若虫中重建了头壳骨骼和肌肉。结果揭示了口针牵引肌出人意料的收缩，并提出了韧皮部汁液吸食的新的取食模型。
