Cellular and Molecular Immunology Lab, Federal University of Health Sciences of Porto Alegre (UFCSPA). Porto Alegre, Brazil.
Laboratory of Molecular and Celular Biology, Graduate Programme of Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, SC, Brazil.
Eur J Sport Sci. 2021 Jul;21(7):1073-1082. doi: 10.1080/17461391.2020.1801850. Epub 2020 Aug 6.
We evaluated the impact of maximal exercise on oxidative stress and DNA damage in peripheral blood mononuclear cells (PBMC) from sedentary and exercised lean and obese men. PBMC were collected before, immediately and 1-h after exercise and exposed to hydrogen peroxide (HO; 25 and 50 µM, 4 h). A leukocytosis was induced by maximal exercise immediately and 1-h after exercise in all groups. However, a lymphopenia was observed 1-h after exercise in the Sedentary obese group. In the control condition, low DNA damage index concomitant to increases in intracellular glutathione content (GSH) was identified immediately after exercise in all groups. However, higher DNA damage index and lipid peroxidation occurred 1-h after the bout in Sedentary and Exercised Obese groups. PBMC exposed to both HO 25 and 50 µM experienced higher DNA damage and lipid peroxidation index immediately after exercise in all groups. Both lipid peroxidation and DNA damage index remained higher in PBMC of Sedentary Lean, Sedentary Obese, and Exercised obese groups obtained 1-h after exercise in both HO 25 and 50 µM, with the highest values identified in PBMC from Sedentary Obese group. However, increases in GSH content were identified in treated PBMC from sedentary and exercised lean groups as well as exercised obese group 1-h after exercise. Habitual exercise confers increased resistance of PBMC to DNA damage induced by oxidative stress, reducing the detrimental effects of obesity.Keywords: Exercise, physical activity, DNA damage, obesity, mutagenesis, oxidative stress.
我们评估了最大运动对久坐和运动的瘦人和肥胖男性外周血单个核细胞（PBMC）中氧化应激和 DNA 损伤的影响。在运动前、运动后即刻和 1 小时采集 PBMC，并将其暴露于过氧化氢（HO；25 和 50µM，4 小时）中。在所有组中，最大运动后即刻和 1 小时后都会引起白细胞增多。然而，在久坐肥胖组中，在运动后 1 小时观察到淋巴细胞减少。在对照条件下，在所有组中，运动后即刻会伴随着细胞内谷胱甘肽（GSH）含量增加而出现低 DNA 损伤指数。然而，在久坐和运动肥胖组中，运动后 1 小时会发生更高的 DNA 损伤指数和脂质过氧化作用。暴露于 25 和 50µM HO 的 PBMC 在所有组中运动后即刻均经历更高的 DNA 损伤和脂质过氧化指数。在 25 和 50µM 的 HO 中，久坐瘦组、久坐肥胖组和运动肥胖组的 PBMC 的脂质过氧化和 DNA 损伤指数在运动后 1 小时后仍然较高，其中在久坐肥胖组的 PBMC 中发现的最高值。然而，在运动后 1 小时，在久坐和运动瘦组以及运动肥胖组的处理 PBMC 中均发现 GSH 含量增加。习惯性运动赋予 PBMC 对氧化应激诱导的 DNA 损伤的更高抵抗力，从而减少肥胖的有害影响。关键词：运动、体力活动、DNA 损伤、肥胖、诱变、氧化应激。
