Wraight C, Hoogenraad N
Department of Biochemistry, La Trobe University, Bundoora.
Aust J Biol Sci. 1988;41(4):435-40. doi: 10.1071/bi9880435.
In the rat, changes in dietary protein intake give rise to changes in the levels of ornithine transcarbamylase (OTC) in liver and small intestine--an increase in liver and decrease in small intestine. The changes in enzyme level are accompanied by similar changes in levels of specific mRNA. Thus in liver, there is an increase in the level of specific mRNA when protein intake is increased, whereas in small intestine there is a small decrease. Comparison of changes in specific mRNA with total poly-A-containing RNA showed that the change in OTC mRNA in liver paralleled the change in total RNA levels. In contrast, in small intestine the small decrease in OTC mRNA levels when protein intake was increased was in the face of an increase in the level of total mRNA. Whereas the level of OTC is 20-fold higher in liver than in small intestine, the mRNA level for the enzyme differs by only 2.5-fold.
在大鼠中，饮食蛋白质摄入量的变化会引起肝脏和小肠中鸟氨酸转氨甲酰酶（OTC）水平的变化——肝脏中增加而小肠中减少。酶水平的变化伴随着特定mRNA水平的类似变化。因此，在肝脏中，当蛋白质摄入量增加时，特定mRNA的水平会升高，而在小肠中则略有下降。将特定mRNA的变化与总含多聚腺苷酸RNA进行比较表明，肝脏中OTC mRNA的变化与总RNA水平的变化平行。相比之下，在小肠中，当蛋白质摄入量增加时，OTC mRNA水平略有下降，而此时总mRNA水平却在上升。尽管肝脏中OTC的水平比小肠中高20倍，但该酶的mRNA水平仅相差2.5倍。
