Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul 120-749, South Korea.
Department of Food and Nutrition, College of Natural Sciences, Chosun University, Gwangju 501-759, South Korea.
J Int Soc Sports Nutr. 2014 Jun 14;11:29. doi: 10.1186/1550-2783-11-29. eCollection 2014.
Postmenopausal women experience adverse physiological changes caused by estrogen deprivation. Here, we hypothesized that the administration of isoflavone, a phytoestrogn, and/or physical exercise could reverse changes in the levels of hepatic enzymes disturbed by loss of estrogen to ameliorate postmenopause-related health problems.
Thirty-week-old female Sprague-Dawley rats were divided into five groups: a sham-operated (SHAM) group, ovariectomized groups on a regular diet with exercise (EXE) and without exercise (OVX), and ovariectomized groups on an isoflavone supplemented diet with (ISO + EXE) and without exercise (ISO). Proteomic tools were employed to identify candidate hepatic proteins that were differentially expressed among the five animal groups.
INMT was detected in the SHAM but not in all of the ovariectomized rats. Seven proteins (PPIA, AKR1C3, ALDH2, PSME2, BUCS1, OTC, and GAMT) were identified to have differential expression among the groups. When compared to the SHAM group, the ovariectomy elevated the levels of PPIA, BUCS1, PSME2, AKR1C3, and GAMT while decreasing ALDH2 and OTC. Among these OVX-induced changes, OVX-increased BUCS1 and GAMT levels were noticeably decreased by ISO or EXE and further greatly down-regulated by ISO + EXE. In the case of PSME2, ISO and EXE further increased OVX-upregulated expression levels but ISO + EXE greatly reduced OVX-increased levels. On the other hand OVX-lowered OTC levels were elevated by ISO, EXE, or ISO + EXE. The protein levels of ALDH2, PPIA, and AKR1C3 were not significantly reverted by ISO, EXE or ISO + EXE.
The combination of an isoflavone diet and exercise partly reversed ovariectomy-induced changes in hepatic protein expression levels. Our data suggest that the combinatory regimen of isoflavone supplementation and exercise may be beneficial to menopausal women through modulating hepatic protein expression profiles.
绝经后妇女由于雌激素缺乏会经历不良的生理变化。在这里，我们假设植物雌激素异黄酮的给药和/或体育锻炼可以逆转因雌激素丧失而扰乱的肝酶水平的变化，从而改善与绝经相关的健康问题。
将 30 周龄的雌性 Sprague-Dawley 大鼠分为五组：假手术（SHAM）组、常规饮食加运动（EXE）和不运动（OVX）的去卵巢组、常规饮食加异黄酮补充（ISO + EXE）和不运动的去卵巢组。采用蛋白质组学工具鉴定 5 组动物中差异表达的候选肝蛋白。
INMT 在 SHAM 组中检测到，但在所有去卵巢大鼠中均未检测到。在各组之间鉴定出 7 种具有差异表达的蛋白质（PPIA、AKR1C3、ALDH2、PSME2、BUCS1、OTC 和 GAMT）。与 SHAM 组相比，卵巢切除术增加了 PPIA、BUCS1、PSME2、AKR1C3 和 GAMT 的水平，同时降低了 ALDH2 和 OTC 的水平。在这些 OVX 诱导的变化中，ISO 或 EXE 显著降低了 OVX 增加的 BUCS1 和 GAMT 水平，ISO + EXE 进一步大大下调了 BUCS1 和 GAMT 水平。对于 PSME2，ISO 和 EXE 进一步增加了 OVX 上调的表达水平，但 ISO + EXE 大大降低了 OVX 增加的水平。另一方面，OVX 降低的 OTC 水平被 ISO、EXE 或 ISO + EXE 升高。ALDH2、PPIA 和 AKR1C3 的蛋白水平没有被 ISO、EXE 或 ISO + EXE 显著逆转。
异黄酮饮食和运动的结合部分逆转了卵巢切除诱导的肝蛋白表达水平的变化。我们的数据表明，异黄酮补充和运动的联合方案可能通过调节肝蛋白表达谱对绝经后妇女有益。
