National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Research Institute for Biomedical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
J Vet Med Sci. 2020 Oct 7;82(9):1376-1378. doi: 10.1292/jvms.20-0387. Epub 2020 Jul 27.
We previously developed a multiplex PCR assay for the differentiation of serovar 1a, 1b, 2 and 5 strains of Erysipelothrix rhusiopathiae. In this study, we analyzed the serovar-defining chromosomal region of a serovar 2 swine isolate, which was PCR-positive for both serovars 1a and 2 by the multiplex PCR assay. Genetic analysis of the chromosomal region revealed that, as in serovar 1a strains, the ERH_1440 gene, which is usually truncated or missing in serovar 2 strains, was intact in this strain. This paper first shows an E. rhusiopathiae serovar 2 strain possessing an intact ERH_1440 gene and suggests that care may be needed when determining the serovar of such rare strains by PCR assay.
我们之前开发了一种用于区分红斑丹毒丝菌血清型 1a、1b、2 和 5 菌株的多重 PCR 检测方法。在这项研究中，我们分析了一株血清型 2 猪分离株的血清型定义染色体区域，该分离株通过多重 PCR 检测方法对血清型 1a 和 2 均呈阳性。对染色体区域的遗传分析表明，与血清型 1a 菌株一样，该菌株中的 ERH_1440 基因完整，而该基因通常在血清型 2 菌株中被截断或缺失。本文首次表明一株红斑丹毒丝菌血清型 2 菌株具有完整的 ERH_1440 基因，并建议在通过 PCR 检测方法确定此类罕见菌株的血清型时需谨慎。
