Mori Yumi, Tada Chika, Fukuda Yasuhiro, Nakai Yutaka
Laboratory of Sustainable Animal Environmental Science, Graduate School of Agricultural Science, Tohoku University.
Research Institute for Bioresource and Biotechnology, Ishikawa Prefectural University.
Microbes Environ. 2020;35(3). doi: 10.1264/jsme2.ME18066.
Sulfur-oxidizing bacterial diversity at the surface of cattle manure was characterized throughout the composting process using a sulfur oxidation gene (soxB) clone library approach. In the mesophilic phase, clones related to the genera Hydrogenophaga and Hydrogenophilus were characteristically detected. In the thermophilic phase, clones related to the genera Hydrogenophaga and Thiohalobacter were predominant. In the cooling phase, the predominant soxB sequences were related to the genus Pseudaminobacter and a new sulfur-oxidizing bacterium belonging to the class Alphaproteobacteria. The present study showed changes in the community composition of sulfur-oxidizing bacteria at the surface of compost throughout the composting process.
采用硫氧化基因（soxB）克隆文库方法，对牛粪堆肥过程中堆肥表面硫氧化细菌的多样性进行了表征。在嗜温阶段，典型地检测到与嗜氢菌属和嗜氢单胞菌属相关的克隆。在嗜热阶段，与嗜氢菌属和硫卤杆菌属相关的克隆占主导地位。在冷却阶段，占主导地位的soxB序列与假氨基杆菌属以及属于α-变形菌纲的一种新的硫氧化细菌有关。本研究表明，在整个堆肥过程中，堆肥表面硫氧化细菌的群落组成发生了变化。
