利用多基因表达系统对转基因植物中链脂肪酸生物合成进行多基因工程改造。
Multigene engineering of medium-chain fatty acid biosynthesis in transgenic by a multigene expression system.
作者信息
Zheng Yusheng, Chen Lizhi, Zhu Zhiyong, Li Dongdong, Zhou Peng
机构信息
College of Tropical Crops, Hainan University, Hainan, 570228 Hainan China.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101 China.
出版信息
3 Biotech. 2020 Aug;10(8):340. doi: 10.1007/s13205-020-02340-z. Epub 2020 Jul 17.
Medium-chain fatty acids (MCFAs) are an ideal feedstock for biodiesel and a range of oleochemical products. In this study, different combinations of , and from coconut ( L.) were coexpressed in transgenic a Cre/LoxP multigene expression system. Transgenic lines expressing different combinations of these genes were designated FL ( + ), FK ( + ) and FLK ( + + ). The homozygous seeds of transgenic expressing high levels of these genes were screened, and their fatty acid composition and lipid contents were determined. Compared with its content in wild-type , the lauric acid (C12:0) content was significantly increased by at least 395%, 134% and 124% in FLK, FL and FK seeds, respectively. Meanwhile, the myristic acid (C14:0) content was significantly increased by at least 383%, 106% and 102% in FL, FLK and FK seeds, respectively, compared to its level in wild-type seeds. Therefore, the FLK plants exhibited the best effects to increase the level of C12:0, and FL expressed the optimal combination of genes to increase the level of 14:0 MCFA.
中链脂肪酸（MCFAs）是生物柴油和一系列油脂化学品的理想原料。在本研究中，来自椰子（椰子属）的不同组合的 、 和 在转基因的Cre/LoxP多基因表达系统中共表达。表达这些基因不同组合的转基因株系被命名为FL（ + ）、FK（ + ）和FLK（ + + ）。筛选出表达这些基因高水平的转基因纯合种子，并测定其脂肪酸组成和脂质含量。与野生型中的含量相比，FLK、FL和FK种子中的月桂酸（C12:0）含量分别显著增加了至少395%、134%和124%。同时，与野生型种子中的水平相比，FL、FLK和FK种子中的肉豆蔻酸（C14:0）含量分别显著增加了至少383%、106%和102%。因此，FLK植株在提高C12:0水平方面表现出最佳效果，而FL表达了提高14:0 MCFA水平的最佳基因组合。
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