Department of Bioengineering, Bilecik Şeyh Edebali University, Bilecik, Turkey.
Department of Chemistry, Kütahya Dumlupınar University, Kütahya, Turkey.
Crit Rev Anal Chem. 2022;52(2):294-313. doi: 10.1080/10408347.2020.1797468. Epub 2020 Jul 26.
Zearalenone (ZEN) is a toxic compound produced by the metabolism of fungi () that threaten the food and agricultural industry belonging to the in foods and feeds. ZEN has toxic effects on human and animal health due to its mutagenicity, teratogenicity, carcinogenicity, nephrotoxicity, immunotoxicity, and genotoxicity. To ensure food safety, rapid, precise, and reliable analytical methods can be developed for the detection of toxins such as ZEN. Different selective molecular diagnostic elements are used in conjunction with different detection strategies to achieve this goal. In this review, the use of electrochemical, colorimetric, fluorometric, refractometric as well as other strategies were discussed for ZEN detection. The success of the sensors in analytical performance depends on the development of receptors with increased affinity to the target. This requirement has been met with different immunoassays, aptamer-assays, and molecular imprinting techniques. The immobilization techniques and analysis strategies developed with the combination of nanomaterials provided high precision, reliability, and convenience in ZEN detection, in which electrochemical strategies perform the best.
玉米赤霉烯酮（ZEN）是一种由真菌代谢产生的有毒化合物，对食品和农业产业造成威胁，存在于食品和饲料中。由于其具有致突变性、致畸性、致癌性、肾毒性、免疫毒性和遗传毒性，ZEN 对人类和动物健康具有毒性作用。为了确保食品安全，可以开发快速、准确、可靠的分析方法来检测 ZEN 等毒素。不同的选择性分子诊断元件与不同的检测策略结合使用，以实现这一目标。在这篇综述中，讨论了电化学、比色法、荧光法、折射法以及其他策略在 ZEN 检测中的应用。传感器在分析性能上的成功取决于对目标具有更高亲和力的受体的开发。不同的免疫测定法、适体测定法和分子印迹技术满足了这一要求。结合纳米材料开发的固定化技术和分析策略在 ZEN 检测中提供了高精度、可靠性和便利性，其中电化学策略表现最佳。
