School of Public Health and Management, Chongqing Medical University, Chongqing 400016, People's Republic of China.
College of Pharmacy, Chongqing Medical University, Chongqing 400016, People's Republic of China.
Biosens Bioelectron. 2019 Mar 15;129:139-146. doi: 10.1016/j.bios.2019.01.012. Epub 2019 Jan 15.
Low molecular weight pollutants from foods have aroused global attention due to their toxicity after long-time exposure. There is an increased demand for appropriate methods to detect these pollutants in foods. In this study, a brand-new type of nano metal-organic coordination polymers (MOCPs) nanocarriers (3D sakura-shaped copper (II) ions@L-glutamic acid (L-Glu)) has been first synthesized. We herein demonstrate a facile chelated method that allows the combination of copper (II) ions and L-Glu. A series of controlled experiments have revealed that the reaction time and the ratio of reactants played the crucial roles in affecting the morphology of the final product. 3D sakura-shaped Cu@L-Glu combined with palladium-platinum nanoparticle (Pd-PtNPs) to obtain Cu@L-Glu/Pd-PtNPs acting as the signal tag, which applied in electrochemical aptasensor for ultrasensitive detection of zearalenone (ZEN). A glassy carbon electrode was first modified with spherical Au-PANI-Au nanohybrids to enhance the conductivity and immobilize more amino modified ZEN aptamer. Cu@L-Glu/Pd-PtNPs were labeled with Complementary DNA (partial matching with ZEN aptamer) to form bioconjugates for signal amplification. After the hybridization reaction of ZEN aptamer and the bioconjugates, a significant electrochemical signal from the catalysis of HO by Cu@L-Glu/Pd-PtNPs can be observed. ZEN competed with bioconjugates for binding to ZEN aptamer, resulting in decreased the electrochemical signal. Chronoamperometry was applied to record the final electrochemical signals. Under optimal conditions, the electrochemical aptasensor exhibited desirable sensitive detection of ZEN with a wide linearity ranging from 1 fg/mL to 100 ng/mL and a relatively low detection limit of 0.45 fg/mL (S/N = 3). Furthermore, the proposed electrochemical aptasensor shows excellent selectivity to the ZEN in the presence of possible interfering substances, and has potential application for ZEN detection in food samples.
由于低分子污染物具有长期暴露后的毒性，因此引起了全球关注。人们对开发适当的方法来检测食品中的这些污染物的需求日益增加。在这项研究中，首次合成了一种全新类型的纳米金属有机配位聚合物（MOCPs）纳米载体（3D 樱花状铜（II）离子@L-谷氨酸（L-Glu））。我们在此展示了一种简便的螯合方法，可使铜（II）离子与 L-Glu 结合。一系列对照实验表明，反应时间和反应物的比例在影响最终产物的形态方面起着至关重要的作用。3D 樱花状的 Cu@L-Glu 与钯-铂纳米粒子（Pd-PtNPs）结合，获得 Cu@L-Glu/Pd-PtNPs 作为信号标签，应用于电化学适体传感器中，用于超灵敏检测玉米赤霉烯酮（ZEN）。首先用球形 Au-PANI-Au 纳米杂化物修饰玻碳电极，以增强导电性并固定更多氨基修饰的 ZEN 适体。Cu@L-Glu/Pd-PtNPs 标记互补 DNA（与 ZEN 适体部分匹配）形成生物缀合物以进行信号放大。在 ZEN 适体与生物缀合物发生杂交反应后，可以观察到 Cu@L-Glu/Pd-PtNPs 对 HO 的催化作用产生的显著电化学信号。ZEN 与生物缀合物竞争与 ZEN 适体结合，导致电化学信号降低。计时安培法用于记录最终的电化学信号。在最佳条件下，电化学适体传感器对 ZEN 表现出良好的灵敏检测性能，线性范围从 1fg/mL 到 100ng/mL，检测限相对较低为 0.45fg/mL（S/N=3）。此外，该电化学适体传感器在存在可能的干扰物质时对 ZEN 表现出优异的选择性，并且在食品样品中 ZEN 的检测中具有潜在的应用价值。
