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全基因组序列资源用于研究坏死型植物病原菌 WPP14。

Complete Genome Sequence Resource for the Necrotrophic Plant-Pathogenic Bacterium WPP14.

机构信息

School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, U.S.A.

Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, NY 14853, U.S.A.

出版信息

Plant Dis. 2021 Jan;105(1):196-198. doi: 10.1094/PDIS-05-20-1059-A. Epub 2020 Nov 17.

DOI:10.1094/PDIS-05-20-1059-A
PMID:32720879
Abstract

spp. are a major cause of loss in vegetable and ornamental plant production. One of these species, , can cause soft rot disease on many plants, particularly potato. These diseases lead to significant economic loss and pose food security threats by reducing crop yields in the field, in transit, and during storage. The Gram-negative enterobacterium . WPP14 is a particularly virulent strain for which there is no available closed genome, limiting the molecular research for this important pathogen. Here, we report a high-quality complete and annotated genome sequence of WPP14. The 4,892,225-bp genome was assembled with Nanopore reads and polished with Illumina reads, yielding 394× and 164× coverage, respectively. This closed genome provides a resource for research on improved detection and biology of , which could translate into improved disease management.

摘要

spp. 是蔬菜和观赏植物生产损失的主要原因之一。其中一种, ,可引起许多植物,特别是马铃薯的软腐病。这些疾病导致重大的经济损失,并通过减少田间、运输中和储存期间的作物产量对粮食安全构成威胁。革兰氏阴性肠杆菌. WPP14 是一种特别致命的菌株,目前没有可用的封闭基因组,限制了对这种重要病原体的分子研究。在这里,我们报告了 WPP14 的高质量完整和注释基因组序列。使用纳米孔读数组装了 4,892,225-bp 基因组,并使用 Illumina 读数进行了润色,分别产生了 394×和 164×的覆盖率。这个封闭的基因组为研究 的改进检测和生物学提供了资源,这可能转化为改进的疾病管理。

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