Fekete Florian J, Marotta Nick J, Liu Xuanyu, Weinert Emily E
Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA, United States.
Graduate Program in Molecular, Cellular, and Integrative Biosciences, Penn State University, University Park, PA, United States.
Front Microbiol. 2023 Jul 7;14:1134742. doi: 10.3389/fmicb.2023.1134742. eCollection 2023.
is an important plant pathogen responsible for the destruction of crops through bacterial soft rot, which is modulated by oxygen (O) concentration. A soluble globin coupled sensor protein, DgcO (also referred to as GCS) is one way through which senses oxygen. DgcO contains a diguanylate cyclase output domain producing c-di-GMP. Synthesis of the bacterial second messenger c-di-GMP is increased upon oxygen binding to the sensory globin domain. This work seeks to understand regulation of function by DgcO at the transcript level. RNA sequencing and differential expression analysis revealed that the deletion of DgcO only affects transcript levels in cells grown under aerobic conditions. Differential expression analysis showed that DgcO deletion alters transcript levels for metal transporters. These results, followed by inductively coupled plasma-mass spectrometry showing decreased concentrations of six biologically relevant metals upon DgcO deletion, provide evidence that a globin coupled sensor can affect cellular metal content. These findings improve the understanding of the transcript level control of O-dependent phenotypes in an important phytopathogen and establish a basis for further studies on c-di-GMP-dependent functions in .
是一种重要的植物病原体，通过细菌性软腐病导致作物受损，而细菌性软腐病受氧气（O）浓度调节。一种可溶性珠蛋白偶联传感器蛋白DgcO（也称为GCS）是感知氧气的一种方式。DgcO包含一个产生环二鸟苷酸（c-di-GMP）的双鸟苷酸环化酶输出结构域。当氧气与传感珠蛋白结构域结合时，细菌第二信使c-di-GMP的合成增加。这项工作旨在了解DgcO在转录水平上对功能的调控。RNA测序和差异表达分析表明，DgcO的缺失仅影响在有氧条件下生长的细胞中的转录水平。差异表达分析表明，DgcO的缺失会改变金属转运蛋白的转录水平。这些结果，随后通过电感耦合等离子体质谱法显示DgcO缺失后六种生物学相关金属的浓度降低，提供了证据表明珠蛋白偶联传感器可以影响细胞金属含量。这些发现增进了对一种重要植物病原体中氧依赖性表型转录水平控制的理解，并为进一步研究中c-di-GMP依赖性功能奠定了基础。
