Nambu J R, Murphy-Erdosh C, Andrews P C, Feistner G J, Scheller R H
Department of Biological Sciences, Stanford University, California 94305.
Neuron. 1988 Mar;1(1):55-61. doi: 10.1016/0896-6273(88)90209-7.
We have purified a 9 amino acid amidated neuropeptide, DPKQDFMRFamide, from whole adult D. melanogaster. This peptide exhibits sequence homology to the molluscan bioactive tetrapeptide FMRFamide and is a novel member of the FMRFamide peptide family. The gene encoding DPKQDFMRFamide has been cloned and characterized. It is present in a single copy per haploid genome, is expressed as a unique 1.7 kb mRNA species, and cytologically maps to 46C on the right arm of chromosome 2. Characterization of a cDNA clone indicates that the precursor protein is 347 amino acids in length and contains 5 copies of DPKQDFMRFamide, as well as 10 additional amidated peptides exhibiting varying degrees of structural relatedness. The Drosophila DPKQDFMRFamide gene and the Aplysia FMRFamide gene are ancestrally related; however, peptides display a higher degree of homology within a species than between species, suggesting intragenic concerted evolution of these neuropeptides.
我们从成年黑腹果蝇整体中纯化出了一种含9个氨基酸的酰胺化神经肽——DPKQDFMRFamide。该肽与软体动物生物活性四肽FMRFamide具有序列同源性，是FMRFamide肽家族的一个新成员。编码DPKQDFMRFamide的基因已被克隆并进行了表征。它在单倍体基因组中以单拷贝形式存在，表达为一种独特的1.7 kb mRNA种类，并且在细胞学上定位于2号染色体右臂的46C处。一个cDNA克隆的表征表明，前体蛋白长度为347个氨基酸，包含5个DPKQDFMRFamide拷贝，以及另外10个具有不同程度结构相关性的酰胺化肽。果蝇的DPKQDFMRFamide基因与海兔的FMRFamide基因在进化上相关；然而，肽在物种内显示出比物种间更高程度的同源性，这表明这些神经肽存在基因内协同进化。
