Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, China.
Res Vet Sci. 2020 Oct;132:312-317. doi: 10.1016/j.rvsc.2020.07.009. Epub 2020 Jul 18.
The CTL immune response mediated by MHC I plays an important role in duck anti-TMUV infection. This study reports the expression, purification and crystallization of a complex of duck MHC class I molecules Anpl-UAA*SD, duck β2-microglobulin (Anpl-β2m) and the polypeptide LRKRQLTVL (LRK9) derived from Tembusu virus (TMUV) NS3. The crystal diffraction resolution is 1.50 Å and belongs to the P6 space group, and the unit cell parameters are a = 82.468, b = 82.468, c = 112.507. The Matthew's constant is calculated to be 2.32 Å Da , and an asymmetric unit contains a complex molecule with a solvent content of 47%. The research lays the foundation for the structure of immune molecules about duck anti-TMUV research.
CTL 免疫反应由 MHC I 介导，在鸭抗 TMUV 感染中发挥重要作用。本研究报告了鸭 MHC I 类分子 Anpl-UAA*SD、鸭β2-微球蛋白（Anpl-β2m）和源自 Tembusu 病毒（TMUV）NS3 的多肽 LRKRQLTVL（LRK9）复合物的表达、纯化和结晶。晶体衍射分辨率为 1.50 Å，属于 P6 空间群，单元晶胞参数为 a=82.468、b=82.468、c=112.507。马修常数计算为 2.32 Å·Da，不对称单位包含一个具有 47%溶剂含量的复合物分子。该研究为鸭抗 TMUV 免疫分子的结构研究奠定了基础。
