Stemple D L, Mahanthappa N K, Anderson D J
Division of Biology, California Institute of Technology, Pasadena 91125.
Neuron. 1988 Aug;1(6):517-25. doi: 10.1016/0896-6273(88)90182-1.
To define further the molecules that control sympathoadrenal differentiation, we have investigated the effects of FGF, NGF, and glucocorticoid on cultured neonatal rat adrenal chromaffin cells. Basic FGF (bFGF), like NGF, induces cell division and neurite outgrowth from these cells. Dexamethasone inhibits neuronal differentiation but not proliferation induced by bFGF. Unlike NGF, bFGF will not support the survival of chromaffin cell-derived sympathetic neurons. However, bFGF induces a dependence on NGF. The overlapping but distinct responses to NGF and bFGF may underlie a sequence of events in sympathetic differentiation. bFGF (or another factor) may act locally in developing ganglia to stimulate mitotic expansion and initial axon outgrowth. Subsequent survival and maturation are then controlled by NGF, which is provided by peripheral targets of innervation. In the adrenal gland, glucocorticoids may permit bFGF to amplify the chromaffin population, while preventing neuronal differentiation.
为了进一步确定控制交感肾上腺分化的分子，我们研究了成纤维细胞生长因子（FGF）、神经生长因子（NGF）和糖皮质激素对培养的新生大鼠肾上腺嗜铬细胞的影响。碱性成纤维细胞生长因子（bFGF）与NGF一样，可诱导这些细胞的细胞分裂和神经突生长。地塞米松可抑制神经元分化，但不抑制bFGF诱导的增殖。与NGF不同，bFGF不能支持嗜铬细胞衍生的交感神经元的存活。然而，bFGF可诱导对NGF的依赖性。对NGF和bFGF重叠但不同的反应可能是交感神经分化一系列事件的基础。bFGF（或另一种因子）可能在发育中的神经节中局部起作用，以刺激有丝分裂扩增和初始轴突生长。随后的存活和成熟则由NGF控制，NGF由神经支配的外周靶标提供。在肾上腺中，糖皮质激素可能允许bFGF扩增嗜铬细胞群体，同时防止神经元分化。
