Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic.
Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 10, 616 00 Brno, Czech Republic.
Bioresour Technol. 2020 Nov;315:123885. doi: 10.1016/j.biortech.2020.123885. Epub 2020 Jul 22.
The aim of this work was to investigate the thermophilic bacterium Schelegelella thermodepolymerans DSM 15344 in terms of its polyhydroxyalkanoates (PHA) biosynthesis capacity. The bacterium is capable of converting various sugars into PHA with the optimal growth temperature of 55 °C; therefore, the process of PHA biosynthesis could be robust against contamination. Surprisingly, the highest yield was gained on xylose. Results suggested that S. thermodepolymerans possess unique xylose metabolism since xylose is utilized preferentially with the highest consumption rate as compared to other sugars. In the genome of S. thermodepolymerans DSM 15344, a unique putative xyl operon consisting of genes responsible for xylose utilization and also for its transport was identified, which is a unique feature among PHA producers. The bacterium is capable of biosynthesis of copolymers containing 3-hydroxybutyrate and also 3-hydroxyvalerate subunits. Hence, S.thermodepolymerans seems to be promising candidate for PHA production from xylose rich substrates.
本工作旨在研究嗜热细菌 Schelegelella thermodepolymerans DSM 15344 的聚羟基烷酸酯（PHA）生物合成能力。该细菌能够将各种糖转化为 PHA，最适生长温度为 55°C；因此，PHA 生物合成过程可以抵抗污染。令人惊讶的是，木糖的产量最高。结果表明，S. thermodepolymerans 具有独特的木糖代谢途径，因为与其他糖相比，木糖的利用率更高，消耗速度也最快。在 S. thermodepolymerans DSM 15344 的基因组中，鉴定出一个独特的假定木糖操纵子，该操纵子包含负责木糖利用及其运输的基因，这是 PHA 生产者的独特特征。该细菌能够生物合成含有 3-羟基丁酸和 3-羟基戊酸单元的共聚物。因此，S. thermodepolymerans 似乎是从富含木糖的底物生产 PHA 的有前途的候选者。
