Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic.
Institute of Chemistry, NAWI Graz, University of Graz, Heinrichstrasse 28/VI, 8010 Graz, Austria; ARENA Arbeitsgemeinschaft für Ressourcenschonende & Nachhaltige Technologien, Inffeldgasse 21b, 11 8010 Graz, Austria.
Bioresour Technol. 2021 Apr;325:124704. doi: 10.1016/j.biortech.2021.124704. Epub 2021 Jan 15.
In this work, the mesophilic bacterium Burkholderia sacchari, the halophilic bacterium Halomonas halophila, and the thermophilic bacterium Schlegelella thermodepolymerans were evaluated with regards to their suitability for polyhydroxyalkanoates (PHA) production from model media mimicking lignocellulose hydrolysates. B. sacchari was capable of utilizing all the tested "model hydrolysates", yielding comparable PHA titers and turning out as very robust against lignocellulose-derived microbial inhibitors. On the contrary, H. halophila reached substantially higher PHA titers on hexoses-rich media, while S. thermodepolymerans preferred media rich in pentoses. Both extremophiles were more sensitive to microbial inhibitors than B. sacchari. Nevertheless, considering substantially higher PHA productivity of both extremophiles even in the presence of microbial inhibitors and also other positive factors associated with utilization of extremophiles, such as the reduced risk of microbial contamination, both H. halophila and S. thermodepolymerans are auspicious candidates for sustainable PHA production from abundantly available, inexpensive lignocelluloses.
在这项工作中，评估了嗜温菌 Burkholderia sacchari、嗜盐菌 Halomonas halophila 和嗜热菌 Schlegelella thermodepolymerans 从模拟木质纤维素水解物的模型介质生产聚羟基烷酸酯 (PHA) 的适宜性。B. sacchari 能够利用所有测试的“模型水解物”，产生可比的 PHA 浓度，并且对木质纤维素衍生的微生物抑制剂具有很强的耐受性。相反，H. halophila 在富含己糖的培养基上达到了更高的 PHA 浓度，而 S. thermodepolymerans 则更喜欢富含戊糖的培养基。与 B. sacchari 相比，这两种极端微生物对微生物抑制剂更敏感。然而，考虑到两种极端微生物即使在存在微生物抑制剂的情况下也具有更高的 PHA 生产力，以及与利用极端微生物相关的其他积极因素，例如降低微生物污染的风险，H. halophila 和 S. thermodepolymerans 都是从丰富、廉价的木质纤维素生产可持续 PHA 的有希望的候选者。
