IHU-Méditerranée Infection, Marseille, France; Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France; Department of Biology, University Yahya Fares of Medea, Algeria; National Veterinary High School, Algiers, Algeria.
IHU-Méditerranée Infection, Marseille, France; Aix Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France; PADESCA Laboratory, Veterinary Science Institute, University Constantine 1, 25100, El Khroub, Algeria.
Ticks Tick Borne Dis. 2020 Sep;11(5):101493. doi: 10.1016/j.ttbdis.2020.101493. Epub 2020 Jun 18.
Coxiella-like bacteria are a large group of yet-to-isolate and characterize bacteria phylogenetically close to the agent of Q fever, Coxiella burnetii, and often associated with ixodid ticks worldwide. This study was designed to assess the presence of Coxiella-like endosymbionts (CLE) in ticks and to describe their genetic diversity in different tick species infesting cattle in Algeria. A total of 765 ticks were collected from three locations. The screening of 20 % of sampled ticks (147/765) exhibited the presence of Coxiella-like in 51.7 % (76/147). The sequencing of partial 16S rRNA and the GroEl genes showed an identity higher than 98 % with different Coxiella-like endosymbionts. The phylogenetic analysis based on the 16S rRNA gene showed the positions of identified Coxiella bacteria. Eleven of the 13 sequences from Rhipicephalus, Dermacentor and Hyalomma ticks were grouped in a distinct clade, the other two each represent an independent clade. This study reported that CLE are prevalent in cattle ticks. Most of the identified Coxiella-like bacteria, from different species of ticks found on cattle, were identical. This may mean that, unlike the currently accepted paradigm, Coxiella-like bacteria are not only tick host-associated, but rather can be transmitted from one tick species to another via the vertebrate host.
考克斯氏体样细菌是一组尚未分离和鉴定的细菌，在系统发育上与 Q 热病原体贝氏考克斯体密切相关，并且经常与世界各地的硬蜱有关。本研究旨在评估蜱中的考克斯氏体样内共生菌（CLE）的存在，并描述它们在侵袭阿尔及利亚牛的不同蜱种中的遗传多样性。从三个地点共收集了 765 只蜱。对 20%的采样蜱（147/765）的筛查显示，51.7%（76/147）的蜱中存在考克斯氏体样。部分 16S rRNA 和 GroEl 基因的测序显示与不同的考克斯氏体样内共生菌具有高于 98%的同一性。基于 16S rRNA 基因的系统发育分析显示了鉴定出的考克斯氏细菌的位置。从硬蜱、璃眼蜱和血蜱中获得的 13 个序列中的 11 个聚在一个独特的分支中，另外 2 个各自代表一个独立的分支。本研究报告称，CLE 在牛蜱中普遍存在。从牛身上发现的不同蜱种中鉴定出的大多数考克斯氏体样细菌是相同的。这可能意味着，与目前公认的模式不同，考克斯氏体样细菌不仅与蜱宿主有关，而且可以通过脊椎动物宿主在不同的蜱种之间传播。
