Campos Samanta S DE, Scherwinski-Pereira Jonny E, Bernd Regina B, Fior Claudimar S, Schwaz Sergio F
Departamento de Horticultura e Silvicultura, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Parque Estação Biológica, Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
An Acad Bras Cienc. 2020;92 Suppl 1:e20181215. doi: 10.1590/0001-3765202020181215. Epub 2020 Jul 27.
Butia odorata is a palm native to southern Brazil and Uruguay, not domesticated, much appreciated for its fruits and economic potential. However, the extractivism and the difficulty of propagation have led to the decline of natural populations. The objective of this work was to prove the possibility of induction of somatic embryogenesis in B. odorata. Mature zygotic embryos were induced in two media, MS and Y3, combined with auxin 2,4-D and picloram in five concentrations (2,4-D: 0, 361.99, 452.49, 542.99 and 633.48 μM/L, picloram: 0, 50, 150, 300 and 450 μM/L). The results promising during induction with the formation of embryogenic calli and somatic embryos, however the regeneration of them was not efficient, this may be due to the occurrence of somatic embryos fused during its development. The roots were formed, but the aerial part remained molten, not completing its development. Auxin picloram and Y3 medium provided the most adequate conditions for calogenesis, formation of embryogenic callus and somatic embryos, with concentrations of 150, 300 and 450 μM/L. This is the first description of somatic embryogenesis in B. odorata that will serve as the basis for future research and adjustments of the methodology proposed here.
香籽酒椰是一种原产于巴西南部和乌拉圭的棕榈树，未被驯化，因其果实和经济潜力而备受青睐。然而，过度采伐和繁殖困难导致了自然种群数量的下降。这项工作的目的是证明诱导香籽酒椰体细胞胚胎发生的可能性。在两种培养基（MS和Y3）中，将成熟合子胚与五种浓度的生长素2,4-D和毒莠定（2,4-D：0、361.99、452.49、542.99和633.48 μM/L，毒莠定：0、50、150、300和450 μM/L）组合进行诱导。诱导过程中形成胚性愈伤组织和体细胞胚的结果很有希望，然而它们的再生效率不高，这可能是由于体细胞胚在发育过程中发生融合。根形成了，但地上部分仍呈熔融状态，未完成其发育。毒莠定和Y3培养基在浓度为150、300和450 μM/L时为愈伤组织形成、胚性愈伤组织和体细胞胚的形成提供了最适宜的条件。这是对香籽酒椰体细胞胚胎发生的首次描述，将为今后的研究和此处提出的方法调整奠定基础。
