Meira Filipe S, Luis Zanderluce G, Cardoso InaÊ MariÊ A S, Scherwinski-Pereira Jonny E
Programa de Pós-Graduação em Biotecnologia e Biodiversidade, Universidade de Brasília/UNB, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Rede Pró-Centro-Oeste, Campus Universitário Darcy Ribeiro, Asa Norte, 70910-900 Brasília, DF, Brazil.
Universidade Federal do Sul e Sudeste do Pará/UNIFESSPA, Instituto de Estudo em Saúde e Biológicas/IESB, Residencial Total Ville, Nova Marabá, 68507-590 Marabá, PA, Brazil.
An Acad Bras Cienc. 2020 Nov 11;92(3):e20180709. doi: 10.1590/0001-3765202020180709. eCollection 2020.
A somatic embryogenesis protocol was developed from the immature leaves of adult plants of the macaw palm. Leaf explants from different regions of the palm heart were used for callus initiation in a modified Y3 medium, supplemented with 2,4-D or Picloram at 450 μM. Calli were separated from the leaf explants at 6-, 9- and 12-month periods and transferred to a fresh culture medium of the same composition. They were multiplied for up to 120 days. Reduced concentrations of 2,4-D and Picloram were used to differentiate somatic embryos. They were then germinated in a medium without plant growth regulators. Morphological and anatomical analyses were conducted at different stages of the embryogenic process. The best results for callus induction were achieved by Picloram, when explants were maintained for up to 9 months on culture medium (64.9%). The farthest portions of the apical meristem were those that provided the biggest calli formation. The formation of the somatic embryos was observed from the calli multiplication phase. Reduction in concentrations of growth regulators failed to promote the formation of complete plants. Picloram at 450 μM promotes high callogenesis in leaf tissues of macaw palm, with a potential for somatic embryo formation.
从金刚鹦鹉棕榈成年植株的未成熟叶片中开发出了一种体细胞胚胎发生方案。使用来自棕榈心不同区域的叶片外植体，在添加了450 μM 2,4 - D或毒莠定的改良Y3培养基中诱导愈伤组织。在6个月、9个月和12个月时将愈伤组织与叶片外植体分离，并转移到相同成分的新鲜培养基中。它们增殖长达120天。使用降低浓度的2,4 - D和毒莠定来诱导体细胞胚胎分化。然后将它们在不含植物生长调节剂的培养基中萌发。在胚胎发生过程的不同阶段进行了形态学和解剖学分析。当外植体在培养基上培养长达9个月时，毒莠定诱导愈伤组织的效果最佳（64.9%）。顶端分生组织最远的部分形成的愈伤组织最大。从愈伤组织增殖阶段观察到了体细胞胚胎的形成。生长调节剂浓度的降低未能促进完整植株的形成。450 μM的毒莠定可促进金刚鹦鹉棕榈叶片组织的高愈伤组织形成，具有体细胞胚胎形成的潜力。
