Zhang Rui-Qian, Wu Xiao-Yi, Hu Tian-Yuan, Song Ya-di, Gao Wei, Huang Lu-Qi
School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China.
School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China School of Pharmaceutical Sciences, Capital Medical University Beijing 100069, China.
Zhongguo Zhong Yao Za Zhi. 2020 Jul;45(13):3098-3103. doi: 10.19540/j.cnki.cjcmm.20200506.105.
Based on the theory of Q-marker, the hairy root of Salvia miltiorrhiza and S. miltiorrhiza in many provinces were studied. The relative expressions of SmCPS, SmKSL and CYP76AH1 genes in hairy roots were detected by real-time fluorescence quantitative PCR and the contents of tanshinoneⅡ_A, cryptotanshinone, tanshinoneⅠ, 1,2-dihydrotanshinone, ferruginol and miltiradiene were detected by UPLC and GC-MS, respectively. Statistical analysis shows as fllows: in the hairy root of S. miltiorrhiza, the content of miltiradiene and ferruginol is positively correlated with the content of tanshinone compounds in the downstream, and the relative expression of important genes in the biosynthetic pathway of tanshinone can reflect the content of tanshinone compounds to a certain extent; in many provinces of S. miltiorrhiza, the content of ferruginol and tanshinone compounds can also be found that there is a positive correlation between the contents. Based on the biosynthetic pathway of tanshinone compounds, which is a special index component in S. miltiorrhiza, this study focused on the important relationship between the upstream gene, the middle intermediate compound and the downstream tanshinone compound content of the biosynthetic pathway, and explored the possible research ideas of improving the quality marker system of S. miltiorrhiza, and then provided the possible research ideas for understanding and studying the quality marker of traditional Chinese medicine from the biosynthetic pathway.
基于Q-标志物理论，对丹参毛状根及多个省份的丹参进行了研究。采用实时荧光定量PCR检测毛状根中SmCPS、SmKSL和CYP76AH1基因的相对表达量，分别采用超高效液相色谱法（UPLC）和气相色谱-质谱联用法（GC-MS）检测丹参酮Ⅱ_A、隐丹参酮、丹参酮Ⅰ、1,2-二氢丹参酮、铁锈醇和丹参烯的含量。统计分析结果如下：在丹参毛状根中，丹参烯和铁锈醇的含量与下游丹参酮类化合物的含量呈正相关，丹参酮生物合成途径中重要基因的相对表达量在一定程度上能反映丹参酮类化合物的含量；在多个省份的丹参中，也发现铁锈醇含量与丹参酮类化合物含量之间存在正相关。基于丹参酮类化合物生物合成途径这一丹参特有的指标性成分，本研究重点关注了生物合成途径上游基因、中间中间体化合物与下游丹参酮类化合物含量之间的重要关系，探索了完善丹参质量标志物体系的可能研究思路，进而为从生物合成途径理解和研究中药质量标志物提供了可能的研究思路。
