Yang Dongfeng, Fang Yumin, Xia Pengguo, Zhang Xiaodan, Liang Zongsuo
Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China.
Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China.
Gene. 2018 Feb 15;643:61-67. doi: 10.1016/j.gene.2017.11.067. Epub 2017 Nov 28.
Salvia miltiorrhiza (S. miltiorrhiza) and Salvia castanea Diels f. tomentosa (S. castanea) are both used for treatment of cardiovascular diseases. They have the same bioactive compound tanshinones, but whose contents are hugely different. This study illustrated diverse responses of tanshinone biosynthesis to yeast extract (YE) and Ag in hairy roots of the two species. YE enhanced both the growth and tanshinone biosynthesis of two hairy roots, and contributed more to tanshinone accumulation in S. castanea than that in S. miltiorrhiza. Genes encoding 1-deoxy-d-xylulose 5-phosphate synthase (DXS2), geranylgeranyl diphosphatesynthase (GGPPS1), copalyl diphosphate synthase (CPS1), and two cytochromes P450 (CYP76AH1 and CYP76AH3) were also more responsive to YE in S. castanea than those in S. miltiorrhiza. Accumulations of dihydrotanshinone I and tanshinone I, and most biosynthetic genes in S. miltiorrhiza were more responsive to Ag than those in S. castanea. Accumulations of dihydrotanshinone I and cryptotanshinone were more responsive to YE, while tanshinone IIA accumulation was more responsive to Ag in S. miltiorrhiza. However, accumulations of other four tanshinones and related genes in S. castanea were more responsive to YE than Ag. This study provides foundations for studying diverse specialized metabolism between the related species.
丹参（Salvia miltiorrhiza）和绒毛鼠尾草（Salvia castanea Diels f. tomentosa）均用于治疗心血管疾病。它们含有相同的生物活性化合物丹参酮，但含量差异巨大。本研究阐明了丹参酮生物合成对这两个物种毛状根中酵母提取物（YE）和银的不同反应。YE促进了两种毛状根的生长和丹参酮生物合成，且对绒毛鼠尾草中丹参酮积累的促进作用比对丹参的更大。编码1-脱氧-D-木酮糖-5-磷酸合酶（DXS2）、香叶基香叶基二磷酸合酶（GGPPS1）、柯巴基二磷酸合酶（CPS1）以及两种细胞色素P450（CYP76AH1和CYP76AH3）的基因在绒毛鼠尾草中对YE的反应也比对丹参的更敏感。丹参中二氢丹参酮I和丹参酮I以及大多数生物合成基因对银的反应比对绒毛鼠尾草中的更敏感。在丹参中，二氢丹参酮I和隐丹参酮的积累对YE更敏感，而丹参酮IIA的积累对银更敏感。然而，绒毛鼠尾草中其他四种丹参酮及其相关基因的积累对YE的反应比对银的更敏感。本研究为研究相关物种间不同的次生代谢提供了基础。
