Community Medicine Department, National Research Centre, Cairo, Egypt.
Child Health Department, National Research Centre, Cairo, Egypt.
PLoS One. 2020 Jul 29;15(7):e0236453. doi: 10.1371/journal.pone.0236453. eCollection 2020.
To assess the potential value of some miRNAs as diagnostic biomarkers for mild cognitive impairment (MCI) among patients with type2 diabetes mellitus (T2DM) and to identify other risk factors for MCI among them.
This study enrolled 163 adults with T2DM using face to face interview. Cognitive function with its domains was assessed using Adenbrooke's Cognitive Examination III (ACE III). Lipid profile, glycated hemoglobin, and miR-128, miR-132, miR- 874, miR-134, miR-323, and miR-382 expressions, using quantitative real-time PCR, were assessed.
MCI was detected among 59/163 (36.2%) patients with T2DM. Plasma expression of miR-132 was significantly higher in T2DM patients with MCI compared to those without MCI and to normal cognitive healthy individuals (median = 2, 1.1 and 1.2 respectively, P < 0.05. Logistic regression analysis showed that higher miR-132 expression with adjusted odds ratio (AOR): 1.2 (95% CI 1.0-1.3), female gender (AOR:2.1; 95%CI 1.0-4.3), education below postgraduate (secondary and university education with AOR: 9.5 & 19.4 respectively) were the significant predicting factors for MCI among T2DM patients. Using ROC curve, miR-132 was the only assayed miRNA that significantly differentiates T2DM patients with MCI from those with normal cognition with 72.3% sensitivity, 56.2% specificity, and 63.8% accuracy (P < 0.05). Other studied miRNAs showed lower sensitivity and specificity for detecting MCI among studied T2DM participants.
MCI affects nearly one-third of adult patients with T2DM. A significantly over expression of miR-132 was detected among T2DM with MCI compared to those with normal cognition.
评估一些 microRNA 作为 2 型糖尿病（T2DM）患者轻度认知障碍（MCI）的诊断生物标志物的潜在价值，并确定其中 MCI 的其他危险因素。
本研究通过面对面访谈纳入了 163 名 T2DM 成年患者。使用阿登布鲁克认知测验 III（ACE III）评估认知功能及其领域。使用定量实时 PCR 评估血脂谱、糖化血红蛋白和 miR-128、miR-132、miR-874、miR-134、miR-323 和 miR-382 的表达。
在 163 例 T2DM 患者中，检测到 59 例（36.2%）MCI。与无 MCI 和认知正常的个体相比，MCI 患者的血浆 miR-132 表达显著升高（中位数分别为 2、1.1 和 1.2，P < 0.05。Logistic 回归分析显示，miR-132 表达较高的调整优势比（AOR）为 1.2（95%CI 1.0-1.3），女性（AOR：2.1；95%CI 1.0-4.3），受教育程度低于研究生（中学和大学教育的 AOR 分别为 9.5 和 19.4）是 T2DM 患者 MCI 的显著预测因素。使用 ROC 曲线，miR-132 是唯一能够显著区分 T2DM 伴 MCI 患者与认知正常患者的检测 miRNA，其敏感性为 72.3%，特异性为 56.2%，准确性为 63.8%（P < 0.05）。其他研究的 miRNA 对检测研究 T2DM 参与者中的 MCI 的敏感性和特异性较低。
MCI 影响近三分之一的 2 型糖尿病成年患者。与认知正常的患者相比，MCI 的 T2DM 患者中 miR-132 的表达显著上调。
