植酸酶处理高粱粉对无麸质食品替代品及必需矿物质生物可利用性的影响
Effect of phytase treatment of sorghum flour, an alternative for gluten free foods and bioaccessibility of essential minerals.
作者信息
Rebellato Ana Paula, Orlando Eduardo A, Thedoropoulos Viviane C Toretti, Greiner Ralf, Pallone Juliana A Lima
机构信息
Department of Food Science, School of Food Engineering, University of Campinas, Monteiro Lobato Street, 80, Campinas, São Paulo 13083-862 Brazil.
Food Technology and Bioprocess Engineering, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany.
出版信息
J Food Sci Technol. 2020 Sep;57(9):3474-3481. doi: 10.1007/s13197-020-04382-w. Epub 2020 Apr 11.
This study evaluated the effect of phytase treatment on the bioavailability of iron (Fe), calcium (Ca), zinc (Zn), and inositol phosphate fractions in sorghum flour; and characterized its macronutrients and minerals. The proximate composition and mineral content indicated that, sorghum flour has a nutritional potential superior to wheat and maize. The results obtained in the solubility and dialysis assays indicated that, naturally occurring minerals (without phytase treatment) in sorghum flour, presented considerable bioaccessibility; reaching 32, 47 and 67% of dialyzable Fe, Zn, and Ca respectively. The use of phytase had a positive influence on the reduction of inositol phosphates, mainly the IP6 fraction, present in sorghum flour samples, and an increase in the soluble percentage (Fe 52% for one sample, for Zn higher than 266%) and dialyzed minerals (Fe 7.8-150%; Zn 19.7 for one sample; and Ca 5-205%) for most samples. Therefore, the essential minerals naturally occurring in sorghum have an absorption potential; and the use of phytase reduced the IP6 fraction and improved the availability of the minerals evaluated.
本研究评估了植酸酶处理对高粱粉中铁（Fe）、钙（Ca）、锌（Zn）以及肌醇磷酸组分生物利用度的影响；并对其常量营养素和矿物质进行了表征。近似组成和矿物质含量表明，高粱粉具有优于小麦和玉米的营养潜力。在溶解度和透析试验中获得的结果表明，高粱粉中的天然矿物质（未进行植酸酶处理）具有相当高的生物可及性；可透析的Fe、Zn和Ca分别达到32%、47%和67%。植酸酶的使用对高粱粉样品中肌醇磷酸（主要是IP6组分）的减少有积极影响，并且大多数样品的可溶百分比（一个样品中Fe为52%，Zn高于266%）和透析矿物质（Fe为7.8 - 150%；一个样品中Zn为19.7；Ca为5 - 205%）有所增加。因此，高粱中天然存在的必需矿物质具有吸收潜力；植酸酶的使用降低了IP6组分并提高了所评估矿物质的利用率。
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